GTF3C6 Antibody - Azide and BSA Free

Immunoprecipitation: GTF3C6 Antibody - Azide and BSA Free [NBP3-23528] - GTF3C6 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate 4 uL NBP3-23528 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-23528, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 34 kDa

Immunohistochemistry-Paraffin: GTF3C6 Antibody - Azide and BSA Free [NBP3-23528] - Immunochemical staining of human GTF3C6 in human skin with NBP3-23528 at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: GTF3C6 Antibody - Azide and BSA Free [NBP3-23528] - Immunochemical staining of human GTF3C6 in human kidney with NBP3-23528 at 1:100 dilution, formalin-fixed paraffin embedded sections.

Western Blot: GTF3C6 Antibody - Azide and BSA Free [NBP3-23528] - NBP3-23528 at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: K-562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 34 kDa

Immunocytochemistry/ Immunofluorescence: GTF3C6 Antibody [NBP3-23528] - Immunofluorescence staining of GTF3C6 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-23528 (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Nucleus.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GTF3C6 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human GTF3C6 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF3C6
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.0
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for GTF3C6 Antibody - Azide and BSA Free

• bA397G5.3
• C6orf51chromosome 6 open reading frame 51
• general transcription factor 3C polypeptide 6
• general transcription factor IIIC, polypeptide 6, alpha 35kDa
• TFIIIC35TFIIIC 35 kDa subunit
• Transcription factor IIIC 35 kDa subunit
• transcription factor IIIC 35kDa
• Transcription factor IIIC subunit 6

Background

RNA polymerases are unable to initiate RNA synthesis in the absence of additional proteins called general transcriptionfactors (GTFs). GTFs assemble in a complex on the DNA promoter and recruit the RNA polymerase. GTF3C family proteins(e.g., GTF3C1, MIM 603246) are essential for RNA polymerase III to make a number of small nuclear and cytoplasmicRNAs, including 5S RNA (MIM 180420), tRNA, and adenovirus-associated (VA) RNA of both cellular and viralorigin.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF3C6