GTF3C6 Antibody - Azide and BSA Free

Western Blot: GTF3C6 Antibody [NBP3-04529] - analysis of various lysates, using GTF3C6 Rabbit pAb at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GTF3C6 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit GTF3C6 Antibody - Azide and BSA Free (NBP3-04529) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human GTF3C6 (NP_612417.1). MAAAADERSPEDGEDEEEEEQLVLVELSGIIDSDFLSKCENKCKVLGIDTERPILQVDSCVFAGEYEDTLGTCVIFEENVEHADTEGNNKTVLKYKCHTMKKLSMTRTLLTEKKEGEENIGGVEWLQIKDNDFSYRPNMICNFLHENEDEEVVASAPDKSLELEEEEIQMNDSSNLSCEQEKPMHLEIEDSGPLIDIPSETEGSVFMETQMLP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF3C6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000 -1:6000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for GTF3C6 Antibody - Azide and BSA Free

• bA397G5.3
• C6orf51chromosome 6 open reading frame 51
• general transcription factor 3C polypeptide 6
• general transcription factor IIIC, polypeptide 6, alpha 35kDa
• TFIIIC35TFIIIC 35 kDa subunit
• Transcription factor IIIC 35 kDa subunit
• transcription factor IIIC 35kDa
• Transcription factor IIIC subunit 6

Background

RNA polymerases are unable to initiate RNA synthesis in the absence of additional proteins called general transcriptionfactors (GTFs). GTFs assemble in a complex on the DNA promoter and recruit the RNA polymerase. GTF3C family proteins(e.g., GTF3C1, MIM 603246) are essential for RNA polymerase III to make a number of small nuclear and cytoplasmicRNAs, including 5S RNA (MIM 180420), tRNA, and adenovirus-associated (VA) RNA of both cellular and viralorigin.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: GTF3C6