GTF3C4 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

GTF3C4 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human GTF3C4.

  Source: E. coli

  Amino Acid Sequence: FWRFKLFLLRILYQSMQKTPSEALWKPTHEDSKILLVDSPGMGNADDEQQEEGTSSKQVVKQGLQERSKEGDVEEPTDDSLPT

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: GTF3C4
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-57486.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for GTF3C4 Recombinant Protein Antigen

• EC 2.3.1.48
• FLJ21002
• general transcription factor 3C 4
• general transcription factor 3C polypeptide 4
• general transcription factor IIIC, polypeptide 4 (90kD)
• general transcription factor IIIC, polypeptide 4, 90kDa
• KAT12
• TF3C-delta
• TFIII90
• TFIIIC 90 kDa subunit
• TFIIIC290
• TFIIIC90MGC138450
• TFIIICDELTA
• Transcription factor IIIC 90 kDa subunit
• Transcription factor IIIC subunit delta

Background

General transcription factor 3C polypeptide 4 (GTF3C4/TFIIIC90) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C4/TFIIIC90 is required for RNA polymerase III transcription and interacts with TFIIIC220, TFIIIC110, TFCIIIC63, TFIIIB90, and RPC39. GTF3C4/TFIIIC90 has been shown to possess acetyltransferase activity specific for histone H3. Alternate names for GTF3C4/TFIIIC90 include transcription factor IIIC subunit delta, TF3C-delta, TFIIIC 90 kDa subunit, FLJ21002, and MGC138450.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GTF3C4