GTF3C4 Antibody

Product Discontinued

GTF3C4 Antibody Summary

• Immunogen: Peptide with sequence C-ERLYETSYRLSKNE corresponding to internal region according to NP_036336.2.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GTF3C4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Preliminary experiments gave an approx. 80 kDa band in K562 lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa band according to NP_036336.2. The 80 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for GTF3C4 Antibody

• EC 2.3.1.48
• FLJ21002
• general transcription factor 3C 4
• general transcription factor 3C polypeptide 4
• general transcription factor IIIC, polypeptide 4 (90kD)
• general transcription factor IIIC, polypeptide 4, 90kDa
• KAT12
• TF3C-delta
• TFIII90
• TFIIIC 90 kDa subunit
• TFIIIC290
• TFIIIC90MGC138450
• TFIIICDELTA
• Transcription factor IIIC 90 kDa subunit
• Transcription factor IIIC subunit delta

Background

General transcription factor 3C polypeptide 4 (GTF3C4/TFIIIC90) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C4/TFIIIC90 is required for RNA polymerase III transcription and interacts with TFIIIC220, TFIIIC110, TFCIIIC63, TFIIIB90, and RPC39. GTF3C4/TFIIIC90 has been shown to possess acetyltransferase activity specific for histone H3. Alternate names for GTF3C4/TFIIIC90 include transcription factor IIIC subunit delta, TF3C-delta, TFIIIC 90 kDa subunit, FLJ21002, and MGC138450.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GTF3C4 Antibody (NBP1-46124)(1)

Publication using NBP1-46124

• Dumay-Odelot H, Marck C, Durrieu-Gaillard S et al. Identification, molecular cloning, and characterization of the sixth subunit of human transcription factor TFIIIC. J Biol Chem 2007-06-01 [PMID: 17409385]

Bioinformatics

• Gene Symbol: GTF3C4
• Entrez:
  • 9329(Human)
  • 269252(Mouse)
  • 685539(Rat)
• Uniprot:
  • NP_036336.2(Human)