GRK5 Antibody (691816)

GRK5 was detected in immersion fixed BJAB human Burkitt's lymphoma cell line using Mouse Anti-Human GRK5 Monoclonal Antibody (Catalog # MAB4539) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 691816
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GRK5 Antibody (691816) Summary

• Immunogen: E. coli-derived recombinant human GRK5
  Pro463-Ser590
  Accession # P34947
• Specificity: Detects human GRK5 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) GRK6 is observed and no cross-reactivity with rhGRK4 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRK5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GRK5 Antibody (691816)

• EC 2.7.11
• EC 2.7.11.16
• FLJ39780
• G protein-coupled receptor kinase 5
• G protein-coupled receptor kinase GRK5
• GPRK5
• GPRK5FP2025
• GRK5

Background

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK5 is a member of the GRK4 subfamily, and is expressed in a wide variety of tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRK5
• Uniprot:
  • P34947()