GPR50 Antibody (461129)

GPR50 was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Mouse Anti-Human GPR50 Monoclonal Antibody (Catalog # MAB4645) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR50 Monoclonal Antibody (Catalog # MAB4645, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Product Discontinued

GPR50 Antibody (461129) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human GPR50
  Met1-Val617
  Accession # Q13585
• Specificity: Detects human GPR50. Stains human GPR50 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPR50
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR50 Antibody (461129)

• G protein-coupled receptor 50H9Mel1c
• GPR50
• H9
• melatonin-related receptor
• MGC125342
• MTR1L

Background

GPR50, also known as MTR1L, is a non-glycosylated seven-transmembrane G protein-coupled receptor that is related to the melatonin receptors MT1 and MT2. GPR50 is expressed in the hippocampus, hypothalamus, and pituitary and forms 130 kDa homodimers. It heterodimerizes with either MT1 or MT2, resulting in inhibition of MT1 but not MT2 function. An alternately spliced isoform of GPR50 has a 4 aa deletion in the large C-terminal cytoplasmic domain. The presence of this deletion as well as various polymorphisms have been associated with elevated serum triglyceride and HDL levels. The deletion may also be associated with the development of bipolar disorder. Human GPR50 shares approximately 70% amino acid sequence identity with mouse and rat GPR50.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPR50
• Uniprot:
  • Q13585()