GPR34 Antibody (419859) [Unconjugated]

GPR34 was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human GPR34 Monoclonal Antibody (Catalog # MAB4617) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR34 Monoclonal Antibody (Catalog # MAB4617, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow, ICC/IF
• Clone: 419859
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GPR34 Antibody (419859) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human GPR34
  Accession # Q9UPC5
• Specificity: Stains human GPR34 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPR34
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR34 Antibody (419859) [Unconjugated]

• G protein-coupled receptor 34
• GPR34
• probable G-protein coupled receptor 34

Background

GPR34 is a widely expressed 75-90 kDa seven transmembrane segment glycoprotein. It is upregulated on microglia during inflammation and functions as a receptor for lysophosphatidyl-L-serine on mast cells to promote degranulation. Multiple isoforms of GPR34 may result from multiple translation initiation sites and alternative splicing. Human GPR34 shares 89% amino acid sequence identity with mouse and rat GPR34.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR34 Antibody (MAB4617)(6)

Publications using MAB4617

• NM Dräger, SM Sattler, CT Huang, OM Teter, K Leng, SH Hashemi, J Hong, G Aviles, CD Clelland, L Zhan, JC Udeochu, L Kodama, AB Singleton, MA Nalls, J Ichida, ME Ward, F Faghri, L Gan, M Kampmann A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states Nature Neuroscience, 2022-08-11;0(0):. 2022-08-11 [PMID: 35953545] (IHC, Human)
• MS Sim, HJ Kim, SH Jo, C Kim, IY Chung Lysophosphatidylserine Induces MUC5AC Production via the Feedforward Regulation of the TACE-EGFR-ERK Pathway in Airway Epithelial Cells in a Receptor-Independent Manner International Journal of Molecular Sciences, 2022-03-31;23(7):. 2022-03-31 [PMID: 35409225] (Flow Cytometry, Human)
• Ruilin Tian, Anthony Abarientos, Jason Hong, Sayed Hadi Hashemi, Rui Yan, Nina Dräger et al. Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis Nature Neuroscience 2021-07-01 [PMID: 34031600]
• H Hasegawa, A Hsu, CE Tinberg, KE Siegler, AA Nazarian, MM Tsai Single amino acid substitution in LC-CDR1 induces Russell body phenotype that attenuates cellular protein synthesis through eIF2? phosphorylation and thereby downregulates IgG secretion despite operational secretory pathway traffic MAbs, 2017-04-05;0(0):0. 2017-04-05 [PMID: 28379093] (ICC, Human)
• Hasegawa H, Patel N, Ettehadieh E, Li P, Lim A Topogenesis and cell surface trafficking of GPR34 are facilitated by positive-inside rule that effects through a tri-basic motif in the first intracellular loop. Biochim Biophys Acta, 2016-04-14;0(0):. 2016-04-14 [PMID: 27086875] (ICC, Human)
• Ansell S, Akasaka T, McPhail E, Manske M, Braggio E, Price-Troska T, Ziesmer S, Secreto F, Fonseca R, Gupta M, Law M, Witzig T, Dyer M, Dogan A, Cerhan J, Novak A t(X;14)(p11;q32) in MALT lymphoma involving GPR34 reveals a role for GPR34 in tumor cell growth. Blood, 2012-09-10;120(19):3949-57. 2012-09-10 [PMID: 22966169] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: GPR34
• Uniprot:
  • Q9UPC5()