GPR164 Overexpression Lysate

Product Discontinued

GPR164 Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC205068 and based on accession number NM_152430. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens olfactory receptor, family 51, subfamily E, member 1 (OR51E1), mRNA.
• Gene: OR51E1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 35.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GPR164 Overexpression Lysate

• D-GPCR
• Dresden-G-protein-coupled receptor
• FLJ13581
• GPR136
• GPR164
• G-protein coupled receptor 164
• MGC24137
• olfactory receptor 51E1
• Olfactory receptor 52A3
• olfactory receptor OR11-15
• olfactory receptor, family 51, subfamily E, member 1 pseudogene
• olfactory receptor, family 51, subfamily E, member 1
• olfactory receptor, family 52, subfamily A, member 3 pseudogene
• OR51E1P
• OR52A3P
• POGR
• prostate overexpressed G protein-coupled receptor
• Prostate-overexpressed G protein-coupled receptor
• Prostate-specific G protein-coupled receptor 2
• PSGR2

Background

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: OR51E1