![Western Blot: ERP27 Antibody [H00121506-B01P] - Analysis of C12orf46 expression in human pancreas.](http://images.novusbio.com/fullsize2/ERP27-Antibody-Western-Blot-H00121506-B01P-img0003.jpg "Western Blot: ERP27 Antibody [H00121506-B01P] - Analysis of C12orf46 expression in human pancreas.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | C12orf46 (NP_689534, 1 a.a. - 273 a.a.) full-length human protein. MEAAPSRFMFLLFLLTCELAAEVAAEVEKSSDGPGAAQEPTWLTDVPAAMEFIAATEVAVIGFFQDLEIPAVPILHSMVQKFPGVSFGISTDSEVLTHYNITGNTICLFRLVDNEQLNLEDEDIESIDATKLSRFIEINSLHMVTEYNPVTVIGLFNSVIQIHLLLIMNKASPEYEENMHRYQKAAKLFQGKILFILVDSGMKENGKVISFFKLKESQLPALAIYQTLDDEWDTLPTAEVSVEHVQNFCDGFLSGKLLKENRESEGKTPKVEL |
| Specificity | C12orf46 - chromosome 12 open reading frame 46, |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Mouse |
| Gene | ERP27 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Immunohistochemistry-Paraffin: Pancreatic Lipase Antibody [NBP2-38958] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/images2/Pancreatic-Lipase-Antibody-Immunohistochemistry-Paraffin-NBP2-38958-img0003.jpg)
![Immunohistochemistry-Paraffin: Pancreatic Lipase Antibody [NBP2-38958] - Staining in human pancreas and tonsil tissues using anti-PNLIP antibody. Corresponding PNLIP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Pancreatic-Lipase-Antibody-Immunohistochemistry-Paraffin-NBP2-38958-img0004.jpg)
![Immunohistochemistry-Paraffin: Pancreatic Lipase Antibody [NBP2-38958] - Staining of human colon, kidney, liver and pancreas using Anti-PNLIP antibody NBP2-38958 (A) shows similar protein distribution across tissues to independent antibody NBP2-38962 (B).](http://images.novusbio.com/images2/Pancreatic-Lipase-Antibody-Immunohistochemistry-Paraffin-NBP2-38958-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TXNDC15 Antibody [NBP1-89394] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.](http://images.novusbio.com/images2/TXNDC15-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89394-img0004.jpg)
![Immunohistochemistry-Paraffin: TXNDC15 Antibody [NBP1-89394] - Staining of human colon shows cytoplasmic positivity with a granular pattern in glandular cells.](http://images.novusbio.com/images2/TXNDC15-Antibody-Immunohistochemistry-Paraffin-NBP1-89394-img0002.jpg)
![Knockdown Validated: Calreticulin Antibody [NB600-101] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/Calreticulin-Antibody-Knockdown-Validated-NB600-101-img0011.jpg)
![Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.](http://images.novusbio.com/images2/Calreticulin-Antibody-Western-Blot-NB600-101-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-101] - The Calreticulin antibody NB600-101 was tested in HeLa cells at a 1:250 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/images2/Calreticulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-101-img0007.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for ERP27 Antibody (H00121506-B01P)Discover more about diseases related to ERP27 Antibody (H00121506-B01P).
| Pathways for ERP27 Antibody (H00121506-B01P)View related products by pathway.
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![Western Blot: ERP27 Antibody [H00121506-B01P] - Analysis of C12orf46 expression in transfected 293T cell line by C12orf46 polyclonal antibody. Lane 1: C12orf46 transfected lysate(30.03 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/ERP27-Antibody-Western-Blot-H00121506-B01P-img0004.jpg "Western Blot: ERP27 Antibody [H00121506-B01P] - Analysis of C12orf46 expression in transfected 293T cell line by C12orf46 polyclonal antibody. Lane 1: C12orf46 transfected lysate(30.03 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: ERp57/PDIA3 Antibody [NBP1-84796] - Analysis using Anti-PDIA3 antibody NBP1-84796 (A) shows similar pattern to independent antibody NBP1-84797 (B).](http://images.novusbio.com/images2/ERp57-PDIA3-Antibody-Western-Blot-NBP1-84796-img0014.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84796] - Staining of human gastrointestinal, prostate, skeletal muscle and thyroid gland using Anti-PDIA3 antibody NBP1-84796 (A) shows similar protein distribution across tissues to independent antibody NBP1-84797 (B).](http://images.novusbio.com/images2/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84796-img0018.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84796] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84796-img0019.jpg)
![Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]](http://images.novusbio.com/images2/Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP1-97019-img0004.jpg "Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]")