ENPP-1 Antibody [Allophycocyanin]

• Applications: Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Allophycocyanin

ENPP-1 Antibody [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ENPP-2-1-2
  Asp49-Trp144 (ENPP-2), Val191-Leu591 (ENPP-1), Asn532-Ile863 (ENPP-2). Cross-reactivity with human ENPP-2 was removed from the final product.
  Accession # Q13822 (ENPP-2) P22413 (ENPP-1)
• Specificity: Detects human ENPP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human ENPP-2 and recombinant mouse ENPP-2 is observed after removal of cross-reactivity with human ENPP-2.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ENPP1
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP-1) is a transmembrane glycoprotein that hydrolyzes nucleotides and nucleotide derivatives with the formation of nucleotide-5’-monophosphates. It is inserted into the plasma membrane by an N‑terminal transmembrane domain. Human ENPP-1 has a small N-terminal cytoplasmic domain and a large C-terminal region containing two somatomedin B-like domains, a catalytic domain and a nuclease-like domain in the extracellular space (1). Defects in the ENPP-1 gene cause arterial calcification and bone mineralization abnormalities (2). ENPP-1 polymorphism or overexpression is also associated with obesity, type II diabetes and insulin resistance, which makes modulation of ENPP-1 activity one of the targets to treat insulin resistance and related diseases (1).

1. Goldfine, I.D. et al. (2010) Endocrine Reviews. 29:62.
2. Hessle, L. et al. (2002) Proc. Natl. Acad. Sci. 99:9445.
 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENPP1