EMR2 Antibodies

4894-EM), immobilized onto a microplate, supports the adhesion of the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human EMR2 (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human EMR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4894). The ND₅₀ is typically 1.5-6 µg/mL." class="big_lightbox"> 4894-EM), immobilized onto a microplate, supports the adhesion of the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human EMR2 (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human EMR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4894). The ND₅₀ is typically 1.5-6 µg/mL." alt="Recombinant Human EMR2 (Catalog # 4894-EM), immobilized onto a microplate, supports the adhesion of the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human EMR2 (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human EMR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4894). The ND₅₀ is typically 1.5-6 µg/mL." class="big_thumb" />

HAF016). A specific band was detected for EMR2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF016). A specific band was detected for EMR2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B)." class="big_lightbox"> MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B)." alt="Human blood‑derived monocytes were stained with Mouse Anti-Human EMR2 Monoclonal Antibody (Catalog # MAB4894, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol." alt="HEK293 human embryonic kidney cell line transfected with (A) human EMR2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human EMR2 Monoclonal Antibody (Catalog # MAB48941) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />