DTYMK Overexpression Lysate (Native)

Images

 
Western Blot: DTYMK Overexpression Lysate (Adult Normal) [NBL1-10039] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DTYMK.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DTYMK Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201228 and based on accession number NM_012145. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens deoxythymidylate kinase (thymidylate kinase) (DTYMK), mRNA.
Gene
DTYMK

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
23.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for DTYMK

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DTYMK Overexpression Lysate (Native)

  • CDC8TMPKTYMKPP3731
  • deoxythymidylate kinase (thymidylate kinase)
  • dTMP kinase
  • EC 2.7.4.9
  • FLJ44192
  • MGC198617
  • thymidylate kinase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-34390
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
NBL1-10259
Species: Hu
NBP1-87441
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-20626
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-87424
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-43639
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-45397
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-46313
Species: Hu, Mk
Applications: WB, IHC, IF
NB100-290
Species: Hu, Mu, Xp
Applications: IP (-), WB, IHC, PLA
NB120-10535
Species: Hu
Applications: WB, ELISA, ICC/IF, IP, MiAr
NBP2-02105
Species: Hu, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-86274
Species: Hu
Applications: WB, IHC, IHC-P, INHIB T-cell
AF4245
Species: Hu
Applications: WB, IP
NBP2-20626
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-59631
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, ICC
NBP1-87822
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-28872
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-55460
Species: Hu
Applications: WB, ICC/IF

Publications for DTYMK Lysate (NBL1-10039) (0)

There are no publications for DTYMK Lysate (NBL1-10039).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DTYMK Lysate (NBL1-10039) (0)

There are no reviews for DTYMK Lysate (NBL1-10039). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DTYMK Lysate (NBL1-10039) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional DTYMK Products

Bioinformatics Tool for DTYMK Lysate (NBL1-10039)

Discover related pathways, diseases and genes to DTYMK Lysate (NBL1-10039). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DTYMK Lysate (NBL1-10039)

Discover more about diseases related to DTYMK Lysate (NBL1-10039).
 

Pathways for DTYMK Lysate (NBL1-10039)

View related products by pathway.

PTMs for DTYMK Lysate (NBL1-10039)

Learn more about PTMs related to DTYMK Lysate (NBL1-10039).
 

Research Areas for DTYMK Lysate (NBL1-10039)

Find related products by research area.

Blogs on DTYMK

There are no specific blogs for DTYMK, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DTYMK Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DTYMK