DPP9 Overexpression Lysate (Native)

Images

 
Western Blot: DPP9 Overexpression Lysate (Adult Normal) [NBL1-10001] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DPP9.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DPP9 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC224465 and based on accession number NM_139159. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens dipeptidyl-peptidase 9 (DPP9), mRNA.
Gene
DPP9

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
96.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for DPP9

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DPP9 Overexpression Lysate (Native)

  • dipeptidyl peptidase 9
  • Dipeptidyl peptidase IV-related protein 2
  • dipeptidyl peptidase IV-related protein-2
  • Dipeptidyl peptidase IX
  • Dipeptidyl peptidase-like protein 9
  • dipeptidylpeptidase 9
  • dipeptidyl-peptidase 9
  • DKFZp762F117
  • DP9
  • DPLP9
  • DPP IX
  • DPP9
  • DPRP2
  • DPRP-2
  • EC 3.4.14.5
  • FLJ16073

Background

This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for DPP9 Lysate (NBL1-10001) (0)

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Bioinformatics

Gene Symbol DPP9
Entrez