![Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (DG1/1487R) [NBP2-53137] - Formalin-fixed, paraffin-embedded human GIST stained with DOG-1 Rabbit Recombinant Monoclonal Antibody (DG1/1487R).](http://images.novusbio.com/fullsize2/DOG1-TMEM16A-Antibody-(DG1-1487R)-Immunohistochemistry-Paraffin-NBP2-53137-img0001.jpg "Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (DG1/1487R) [NBP2-53137] - Formalin-fixed, paraffin-embedded human GIST stained with DOG-1 Rabbit Recombinant Monoclonal Antibody (DG1/1487R).")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IHC-P |
| Clone | DG1/1487R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant full-length human DOG-1 protein |
| Localization | Cell Surface and Cytoplasm |
| Marker | Gastrointestinal Stromal Tumor Marker |
| Protein/Peptide Type | Recombinant Protein |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ANO1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | IHC-P:Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes |
| Theoretical MW | 114 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-54326AF350 | |
| Alexa Fluor 405 | NBP2-54326AF405 | |
| Alexa Fluor 488 | NBP2-54326AF488 | |
| Alexa Fluor 532 | NBP2-54326AF532 | |
| Alexa Fluor 594 | NBP2-54326AF594 | |
| Alexa Fluor 647 | NBP2-54326AF647 | |
| Alexa Fluor 700 | NBP2-54326AF700 | |
| Alexa Fluor 750 | NBP2-54326AF750 | |
| Allophycocyanin | NBP2-54326APC | |
| Biotin | NBP2-54326B | |
| DyLight 350 | NBP2-54326UV | |
| DyLight 405 | NBP2-54326V | |
| DyLight 488 | NBP2-54326G | |
| DyLight 550 | NBP2-54326R | |
| DyLight 594 | NBP2-54326DL594 | |
| DyLight 650 | NBP2-54326C | |
| DyLight 680 | NBP2-54326FR | |
| DyLight 755 | NBP2-54326IR | |
| FITC | NBP2-54326F | |
| HRP | NBP2-54326H | |
| Janelia Fluor 549 | NBP2-54326JF549 | |
| Janelia Fluor 646 | NBP2-54326JF646 | |
| PE | NBP2-54326PE | |
| PerCP | NBP2-54326PCP |
Diseases for DOG1/TMEM16A Antibody (NBP2-53137)Discover more about diseases related to DOG1/TMEM16A Antibody (NBP2-53137).
| Pathways for DOG1/TMEM16A Antibody (NBP2-53137)View related products by pathway.
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PTMs for DOG1/TMEM16A Antibody (NBP2-53137)Learn more about PTMs related to DOG1/TMEM16A Antibody (NBP2-53137).
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ANO1 |
![Immunohistochemistry-Paraffin: CLCA1 Antibody [NBP2-38869] - Staining of human colon.](http://images.novusbio.com/images2/CLCA1-Antibody-Immunohistochemistry-Paraffin-NBP2-38869-img0007.jpg)
![Immunohistochemistry: CLCA1 Antibody [NBP2-38869] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells / Goblet cells strongly stained.](http://images.novusbio.com/images2/CLCA1-Antibody-Immunohistochemistry-NBP2-38869-img0001.jpg)
![Immunohistochemistry-Paraffin: CLCA1 Antibody [NBP2-38869] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/images2/CLCA1-Antibody-Immunohistochemistry-Paraffin-NBP2-38869-img0002.jpg)
![Western Blot: CLCA4 Antibody [NBP2-92365] - Analysis of extracts of Rat uterus, using CLCA4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/images2/CLCA4-Antibody-Western-Blot-NBP2-92365-img0001.jpg)
![Western Blot: CLCA4 Antibody [NBP2-92365] - Analysis of extracts of various cell lines, using CLCA4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/images2/CLCA4 Antibody-Western Blot-NBP2-92365-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CLCA2 Antibody [NBP2-33482] - Staining of human cell line HaCaT shows localization to nucleus & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/images2/CLCA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33482-img0010.jpg)
![Immunohistochemistry-Paraffin: CLCA2 Antibody [NBP2-33482] - Staining of human stomach shows no positivity in glandular cells as expected.](http://images.novusbio.com/images2/CLCA2-Antibody-Immunohistochemistry-Paraffin-NBP2-33482-img0019.jpg)
![Immunohistochemistry-Paraffin: CLCA2 Antibody [NBP2-33482] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/images2/CLCA2-Antibody-Immunohistochemistry-Paraffin-NBP2-33482-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/CD34 Antibody (MEC 14.7)-Immunocytochemistry-Immunofluorescence-NB600-1071-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Immunohistochemistry-Paraffin-NB600-1071-img0014.jpg)
![Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - An intracellular stain was performed on Raw264.7 cells with CD34 Antibody [MEC 14.7] NB600-1071 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488 (SA5-10018, Thermo Fisher).](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Flow-Cytometry-NB600-1071-img0015.jpg)
![Western Blot: ANO6 Antibody [NBP1-59655] - OVCAR-3 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/images2/ANO6 Antibody-Western Blot-NBP1-59655-img0002.jpg)
![Dual RNAscope ISH-IHC: Bestrophin 1 Antibody (E6-6) [NB300-164] - Formalin-fixed paraffin-embedded tissue sections of human duodenum were probed for Bestrophin 1 mRNA (ACD RNAScope Probe, catalog # 433181; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human (Novus Biologicals catalog # NB300-164) at 0.3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). IHC signal is confined to cytoplasm.](http://images.novusbio.com/images2/Bestrophin-1-Antibody-(E6-6)-Dual-RNAscope-ISH-IHC-NB300-164-img0006.jpg)
![Immunohistochemistry-Paraffin: Bestrophin 1 Antibody (E6-6) [NB300-164] - Bestrophin 1 was detected in immersion fixed paraffin sections of human small intestine using t Mouse Anti-Human Bestrophin 1 Monoclonal Antibody (Catalog # NB300-164) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface and extracellular.](http://images.novusbio.com/images2/Bestrophin-1-Antibody-(E6-6)-Immunohistochemistry-Paraffin-NB300-164-img0005.jpg)
![Western Blot: Bestrophin 1 Antibody (E6-6) [NB300-164] - Western blot analysis of the normal and mutant human Best1 protein in transiently transfected MDCK cells. Best1 proteins are detectable as a 68 kDa band in all transfected cells, but not in non-transfected controls (MDCK lane). Actin bands are shown to indicate equal loading of cell lysates. Image collected and cropped by CiteAb from the following publication (http://www.mdpi.com/1422-0067/14/7/15121), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Bestrophin-1-Antibody-(E6-6)-Western-Blot-NB300-164-img0007.jpg)
![Western Blot: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - Analysis of GNAI1 expression in transfected 293T cell line by GNAI1 monoclonal antibody (M01), clone 2B8-2A5.Lane 1: GNAI1 transfected lysate(40.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/G-protein-alpha-inhibitor-1-Antibody-(2B8-2A5)-Western-Blot-H00002770-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - Analysis of monoclonal antibody to GNAI1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/images2/G-protein-alpha-inhibitor-1-Antibody-(2B8-2A5)-Immunohistochemistry-Paraffin-H00002770-M01-img0004.jpg)
![Western Blot: G protein alpha inhibitor 1 Antibody (2B8-2A5) [H00002770-M01] - GNAI1 monoclonal antibody (M01), clone 2B8-2A5. Analysis of GNAI1 expression in human liver.](http://images.novusbio.com/images2/G-protein-alpha-inhibitor-1-Antibody-(2B8-2A5)-Western-Blot-H00002770-M01-img0006.jpg)
![Western Blot: TMEM16K Antibody [NBP1-59667] - Human Adult Placenta, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/images2/TMEM16K-Antibody-Western-Blot-NBP1-59667-img0003.jpg)
![Western Blot: TMEM16K Antibody [NBP1-59667] - 293T cells lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/images2/TMEM16K-Antibody-Western-Blot-NBP1-59667-img0002.jpg)
![Western Blot: TMEM16K Antibody [NBP1-59667] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/images2/TMEM16K Antibody-Western Blot-NBP1-59667-img0004.jpg)
![Western Blot: ANO2 Antibody [NBP2-81703] - Western blot analysis of ANO2 in rat brain tissue lysate with ANO2 antibody at (A) 1 and (B) 2 ug/mL](http://images.novusbio.com/images2/ANO2-Antibody-Western-Blot-NBP2-81703-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ANO2 Antibody [NBP2-81703] - Immunofluorescence of ANO2 in Human Brain cells with ANO2 antibody at 20 ug/mL.](http://images.novusbio.com/images2/ANO2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-81703-img0003.jpg)
![Immunohistochemistry: ANO2 Antibody [NBP2-81703] - Immunohistochemistry of ANO2 in human brain tissue with ANO2 antibody at 5 ug/mL.](http://images.novusbio.com/images2/ANO2-Antibody-Immunohistochemistry-NBP2-81703-img0002.jpg)
