DHX33 Antibody

Western Blot: DHX33 Antibody [NB100-2581] - Detection of Human and Mouse DHX33 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX33 antibody NB100-2581 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: DHX33 Antibody [NB100-2581] - Detection of human DHX33 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DHX33 antibody NB100-2581 (lot 2) used for IP at 6 ug per reaction. DHX33 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-DHX33 antibody NB100-2580. For blotting immunoprecipitated DHX33, NB100-2581 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

DHX33 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 650 and the C-terminus (residue 707) of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 33 using the numbering given in entry NP_064547.2 (GeneID 56919).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DHX33
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: ICC/IF reactivity reported in (PMID: 21930779).
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Mouse reactivity reported in (PMID: 21930779).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DHX33 Antibody

• DDX33
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 33
• DEAH (Asp-Glu-Ala-His) box polypeptide 33
• DEAH box protein 33
• DKFZp762F2011
• EC 3.6.1
• EC 3.6.4.13
• FLJ21972
• putative ATP-dependent RNA helicase DHX33

Background

DHX33 is a DEAD box protein whose function has not yet been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DHX33 Antibody (NB100-2581)(5)

Publications using NB100-2581

• Fraile JM, Campos-Iglesias D, Rodriguez F et al. Loss of the Deubiquitinase USP36 Destabilizes the RNA Helicase DHX33 and Causes Preimplantation Lethality in Mice. J. Biol. Chem. 2017-12-22 [PMID: 29273634] (WB, Mouse)
• Zhang Y, You J, Wang X, Weber J. The DHX33 RNA helicase promotes mRNA translation initiation Mol. Cell. Biol. 2015-06-22 [PMID: 26100019] (WB, Human)
• Weng L, Mitoma H, Tricot C et al. The E3 Ubiquitin Ligase Tripartite Motif 33 Is Essential for Cytosolic RNA-Induced NLRP3 Inflammasome Activation. J Immunol. 2014-10-01 [PMID: 25172487]
• Mitoma H, Hanabuchi S, Kim T, et al. The DHX33 RNA Helicase Senses Cytosolic RNA and Activates the NLRP3 Inflammasome. Immunity 2013-07-25 [PMID: 23871209] (IP, Human)
• Zhang Y, Forys JT, Miceli AP et al. Identification of DHX33 as a mediator of rRNA synthesis and cell growth. Mol Cell Biol 2011-12-01 [PMID: 21930779] (ICC/IF, WB, Human, Mouse)

Bioinformatics

• Gene Symbol: DHX33
• Entrez:
  • 56919(Human)
• Uniprot:
  • NP_064547.2(Human)