DHX33 Antibody - BSA Free

Immunohistochemistry: DHX33 Antibody [NB100-2580] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DHX33 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).

Immunohistochemistry: DHX33 Antibody [NB100-2580] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250. Detection by DAB staining. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.

Immunohistochemistry: DHX33 Antibody [NB100-2580] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.

Immunohistochemistry-Paraffin: DHX33 Antibody [NB100-2580] - Human lung carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).

Immunohistochemistry: DHX33 Antibody [NB100-2580] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DHX33 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).

Immunoprecipitation: DHX33 Antibody [NB100-2580] - Detection of Human DHX33 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DHX33 antibody NB100-2580 used for IP at 3 mcg/mg lysate. DHX33 was also immunoprecipitated by rabbit anti-DHX33 antibody NB100-2581. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DHX33 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DHX33 Antibody - BSA Free (NB100-2580) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 33 using the numbering given in entry NP_064547.2 (GeneID 56919).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DHX33
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DHX33 Antibody - BSA Free

• DDX33
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 33
• DEAH (Asp-Glu-Ala-His) box polypeptide 33
• DEAH box protein 33
• DKFZp762F2011
• EC 3.6.1
• EC 3.6.4.13
• FLJ21972
• putative ATP-dependent RNA helicase DHX33

Background

DHX33 is a DEAD box protein whose function has not yet been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DHX33
• Entrez:
  • 56919(Human)
• Uniprot:
  • Q9H6R0(Human)