DDX31 Antibody - Azide and BSA Free

Western Blot: DDX31 Antibody [NBP2-92273] - Analysis of extracts of A-549 cells, using DDX31 .Exposure time: 3min.

Immunocytochemistry/Immunofluorescence: DDX31 Antibody [NBP2-92273] - Analysis of L929 cells using DDX31 at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: DDX31 Antibody [NBP2-92273] - Analysis of HeLa cells using DDX31 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DDX31 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit DDX31 Antibody - Azide and BSA Free (NBP2-92273) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 652-851 of human DDX31 (NP_073616.6). EIKMEDILCVLTRDDCFKGKRWGAQKSHAVGPQEIRERATVLQTVFEDYVHSSERRVSWAKKALQSFIQAYATYPRELKHIFHVRSLHLGHVAKSFGLRDAPRNLSALTRKKRKAHVKRPDLHKKTQSKHSLAEILRSEYSSGMEADIAKVKKQNAPGEPGGRPLQHSLQPTPCFGRGKTLKWRKTQKGVQRDSKTSQKV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX31
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 94 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for DDX31 Antibody - Azide and BSA Free

• DEAD (Asp-Glu-Ala-Asp) box polypeptide 31
• DEAD box protein 31
• DEAD/DEXH helicase DDX31
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 31
• EC 3.6.1
• EC 3.6.4.13
• FLJ13633
• FLJ14578
• FLJ23349
• G2 helicase
• helicain
• probable ATP-dependent RNA helicase DDX31

Background

DDX31 is a member of DEAD box proteins family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The function of this DDX31 has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: DDX31