DDX28 Antibody

Images

 
Western Blot: DDX28 Antibody [NBP2-16131] - Sample (30 ug of whole cell lysate) A:IMR32 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: DDX28 Antibody [NBP2-16131] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.73 mg/ml

Order Details

DDX28 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DDX28. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX28
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Bovine (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.73 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDX28 Antibody

  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 28
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 28
  • EC 3.6.1
  • EC 3.6.4.13
  • FLJ11282
  • M
  • MDDX28probable ATP-dependent RNA helicase DDX28
  • Mitochondrial DEAD box protein 28
  • mitochondrial DEAD-box polypeptide 28

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DDX28
Uniprot