DDX28 Antibody - BSA Free

Western Blot: DDX28 Antibody [NBP2-16131] - Sample (30 ug of whole cell lysate) A:IMR32 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: DDX28 Antibody [NBP2-16131] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DDX28 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DDX28 Antibody - BSA Free (NBP2-16131) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DDX28. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX28
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for DDX28 Antibody - BSA Free

• DEAD (Asp-Glu-Ala-Asp) box polypeptide 28
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 28
• EC 3.6.1
• EC 3.6.4.13
• FLJ11282
• M
• MDDX28probable ATP-dependent RNA helicase DDX28
• Mitochondrial DEAD box protein 28
• mitochondrial DEAD-box polypeptide 28

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DDX28
• Uniprot:
  • Q9NUL7(Human)