Recombinant Human DCUN1D2 His Protein

SDS-Page: Recombinant Human DCUN1D2 Protein [NBP1-72402] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human DCUN1D2 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-259 of Human DCUN1D2
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MHKLKSSQKD KVRQFMACTQ AGERTAIYCL TQNEWRLDEA TDSFFQNPDS LHRESMRNAV DKKKLERLYG RYKDPQDENK IGVDGIQQFC DDLSLDPASI SVLVIAWKFR AATQCEFSRK EFLDGMTELG CDSMEKLKAL LPRLEQELKD TAKFKDFYQF TFTFAKNPGQ KGLDLEMAVA YWKLVLSGRF KFLDLWNTFL MEHHKRSIPR DTWNLLLDFG NMIADDMSNY DEEGAWPVLI DDFVEYARPV VTGGKRSLF

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DCUN1D2
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 32.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 30% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human DCUN1D2 His Protein

• C13orf17
• chromosome 13 open reading frame 17
• DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae)
• DCN1-like protein 2
• DCUN1 domain-containing protein 2
• DCUN1L2
• Defective in cullin neddylation protein 1-like protein 2
• FLJ10704
• FLJ20092

Background

DCN1-like protein 2, also known as DCUN1D2, which contains one DCUN1 domain and 1 UBA-like domain, may play a role in the neddylation of cullins that regulate SCF-type ubiquitin ligase complexes. The gene encoding Dcun1D2 exists on human chromosome 13. Chromosome 13 houses key tumor suppressor genes, including BRCA2 and RB1, which are associated with breast cancer susceptibility and retinoblastoma, respectively. Recombinant human DCUN1D2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DCUN1D2