CIP29 Overexpression Lysate (Native)

Western Blot: CIP29 Overexpression Lysate (Adult Normal) [NBL1-09210] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CIP29.

• Reactivity: Hu
• Applications: WB

CIP29 Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC203324 and based on accession number NM_033082. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens cytokine induced protein 29 kDa (CIP29), transcript variant 1, mRNA.
• Gene: SARNP

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 23.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for CIP29

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CIP29 Overexpression Lysate (Native)

• CIP29Hcc-1
• cytokine induced protein 29 kDa
• Cytokine-induced protein of 29 kDa
• HCC1MGC14726
• hepatocellular carcinoma 1
• HSPC316
• Nuclear protein Hcc-1
• proliferation associated cytokine-inducible protein CIP29
• Proliferation-associated cytokine-inducible protein CIP29
• SAP domain containing ribonucleoprotein
• SAP domain-containing ribonucleoprotein
• THO1

Background

This gene encodes a protein that is upregulated in response to various cytokines. The encoded protein may play a role in cell cycle progression. A translocation between this gene and the myeloid/lymphoid leukemia gene, resulting in expression of a chimeric protein, has been associated with acute myelomonocytic leukemia. Pseudogenes exist on chromosomes 7 and 8. Alternatively spliced transcript variants have been described. [provided by RefSeq]. Transcript Variant: This variant (1) represents the shortest transcript and encodes the functional protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SARNP