![Immunocytochemistry/Immunofluorescence: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CHCHD5.](http://images.novusbio.com/fullsize/CHCHD5-Antibody-OTI3H3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-72289-img0003.jpg "Immunocytochemistry/Immunofluorescence: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CHCHD5.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI3H3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Description | Novus Biologicals Mouse CHCHD5 Antibody (OTI3H3) - Azide and BSA Free (NBP2-03608) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-110 of human CHCHD5(NP_115685) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CHCHD5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 12.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CHCHD5 |
![Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of paraffin-embedded Human lymphoma tissue using anti-CHCHD5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CHCHD5-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72289-img0008.jpg "Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of paraffin-embedded Human lymphoma tissue using anti-CHCHD5 mouse monoclonal antibody.")
![Flow Cytometry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CHCHD5 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CHCHD5-Antibody-OTI3H3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72289-img0002.jpg "Flow Cytometry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CHCHD5 antibody, and then analyzed by flow cytometry.")
![Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CHCHD5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CHCHD5-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72289-img0004.jpg "Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CHCHD5 mouse monoclonal antibody.")
![Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CHCHD5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CHCHD5-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72289-img0005.jpg "Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CHCHD5 mouse monoclonal antibody.")
![Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of paraffin-embedded Human endometrium tissue using anti-CHCHD5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CHCHD5-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72289-img0006.jpg "Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of paraffin-embedded Human endometrium tissue using anti-CHCHD5 mouse monoclonal antibody.")
![Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of paraffin-embedded Human Kidney tissue using anti-CHCHD5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CHCHD5-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72289-img0007.jpg "Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of paraffin-embedded Human Kidney tissue using anti-CHCHD5 mouse monoclonal antibody.")
![Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of paraffin-embedded Human tonsil using anti-CHCHD5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CHCHD5-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72289-img0009.jpg "Immunohistochemistry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - Staining of paraffin-embedded Human tonsil using anti-CHCHD5 mouse monoclonal antibody.")
![Flow Cytometry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHCHD5.](http://images.novusbio.com/fullsize/CHCHD5-Antibody-OTI3H3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72289-img0001.jpg "Flow Cytometry: CHCHD5 Antibody (OTI3H3) - Azide and BSA Free [NBP2-72289] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHCHD5.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")