CHCHD5 Antibody (OTI3H3)

Images

 
Western Blot: CHCHD5 Antibody (3H3) [NBP2-03608] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: CHCHD5 Antibody (3H3) [NBP2-03608] Staining of COS7 cells transiently transfected by pCMV6-ENTRY CHCHD5.
Immunohistochemistry-Paraffin: CHCHD5 Antibody (3H3) [NBP2-03608] - Staining of paraffin-embedded Human tonsil using anti-CHCHD5 mouse monoclonal antibody.
Flow Cytometry: CHCHD5 Antibody (3H3) [NBP2-03608] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHCHD5 antibody, and then ...read more
Immunohistochemistry-Paraffin: CHCHD5 Antibody (3H3) [NBP2-03608] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CHCHD5 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CHCHD5 Antibody (3H3) [NBP2-03608] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CHCHD5 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CHCHD5 Antibody (3H3) [NBP2-03608] - Staining of paraffin-embedded Human endometrium tissue using anti-CHCHD5 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CHCHD5 Antibody (3H3) [NBP2-03608] - Staining of paraffin-embedded Human Kidney tissue using anti-CHCHD5 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CHCHD5 Antibody (3H3) [NBP2-03608] - Staining of paraffin-embedded Human lymphoma tissue using anti-CHCHD5 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI3H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.35 mg/ml

Order Details

CHCHD5 Antibody (OTI3H3) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-110 of human CHCHD5(NP_115685) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHCHD5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200
Theoretical MW
12.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.35 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CHCHD5 Antibody (OTI3H3)

  • C2orf9
  • chromosome 2 open reading frame 9
  • coiled-coil-helix-coiled-coil-helix domain containing 5
  • coiled-coil-helix-coiled-coil-helix domain-containing protein 5
  • FLJ39671
  • MGC11104

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CHCHD5
Entrez