Carbohydrate Sulfotransferase 7/CHST7 Antibody [Alexa Fluor™ Plus 405]

• Reactivity: Mu
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 405

Carbohydrate Sulfotransferase 7/CHST7 Antibody [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects mouse Sulfotransferase 7/CHST7 in direct ELISAs and Western blots. In direct ELISA, less than 1% cross-reactivity with recombinant mouse (rm) CHST1, recombinant human CHST2, rmCHST5, and rmCHST3 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbohydrate Sulfotransferase 7/CHST7 Antibody [Alexa Fluor™ Plus 405]

• C6ST2
• carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7
• Carbohydrate Sulfotransferase 7
• CHST7
• GlcNAc6ST-4
• GST-5

Background

The CHST family is comprised of 14 genes in both human and mouse. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST7, also designated as chondroitin 6-O-sulfotransferase (C6ST-2), N-acetylglucosamine-6-O-sulfotransferase (GlcNAc6ST-4) and Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST-5), was previously shown to act on chondroitin sulfate, mannose-linked GlcNAc and mucin associated GlcNAc residues (3‑5). The amino acid sequence of mouse CHST7 is 87% identical to that of the human counterpart.

1. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
2. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
3. Kitagawa, H. et al. (2000) J. Biol. Chem. 275:21075.
4. Uchimura, K. et al. (2000) Biochem. Biophys. Res. Commun. 274:291.
5. Bhakta, S. et al. (2000) J. Biol. Chem. 275:40226.
6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, p.964.
7. MacRae, I.J. et al. (2000) Biochemistry 39:1613.
8. Wu, Z.L. et al. (2002) FASEB J. 16:539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.