Carbohydrate Sulfotransferase 5/CHST5 Antibody

Product Discontinued

Carbohydrate Sulfotransferase 5/CHST5 Antibody Summary

• Immunogen: Peptide with sequence C-DSFKWASSTEKQPE corresponding to C-Terminus according to Uniprot Mouse NP_064334.1.
• Epitope: C Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CHST5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave bands at approx. 38 kDa band and 35 kDa band in mouse small intestine lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 44.5 kDa band according to NP_064334.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Carbohydrate Sulfotransferase 5/CHST5 Antibody

• carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5
• Carbohydrate Sulfotransferase 5
• CHST5
• GlcNAc6ST3
• gn6st-3
• GST4-alpha
• hIGn6ST
• I-GlcNAc6ST

Background

CHST5 is a gene that codes for a protein with two isoforms, measuring 411 and 417 amino acids in length, with weights of approximately 46 and 47 kDa respectively. CHST5 functions as a sulfotransferase that does not transfer sulfate to longer carbohydrate structures nor does it have activity toward keratin. Current research is being done of several diseases and disorders related to this gene including corneal dystrophy, adenocarcinoma, esophagitis, cholesterol, immunodeficiency, and hepatitis. CHST5 has also been shown to have interactions with CHST1, KERA, LUM, ST#GAL3, and FMOD in pathways such as the metabolism, Maroteaux-Lamy syndrome, and Hunter syndrome pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carbohydrate Sulfotransferase 5/CHST5 Antibody (NBP1-36947)(1)

Publication using NBP1-36947

• Kitayama K, Hayashida Y, Nishida K, Akama TO. Enzymes responsible for synthesis of corneal keratan sulfate glycosaminoglycans. J Biol Chem 2007-10-01 [PMID: 17690104]

Bioinformatics

• Gene Symbol: CHST5
• Entrez:
  • 56773(Mouse)
  • 307859(Rat)
• Uniprot:
  • NP_064334.1(Mouse)