Carbohydrate Sulfotransferase 2/CHST2 Antibody [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

Carbohydrate Sulfotransferase 2/CHST2 Antibody [Alexa Fluor® 594] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CHST2
  Tyr76-Leu530
  Accession # Q9Y4C5
• Specificity: Detects human Carbohydrate Sulfotransferase 2/CHST2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) CHST1, rhCHST5, and recombinant mouse CHST7 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbohydrate Sulfotransferase 2/CHST2 Antibody [Alexa Fluor® 594]

• C6ST
• carbohydrate (chondroitin 6/keratan) sulfotransferase 2
• carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2
• Carbohydrate Sulfotransferase 2
• CHST2
• EC 2.8.2.-
• Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2
• GlcNAc6ST-1
• GN6ST
• Gn6ST-1
• GST2
• GST-2
• N-acetylglucosamine 6-O-sulfotransferase 1

Background

The CHST family is comprised of 14 genes in both human and mouse. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). Human CHST2, also known as N-acetylglucosamine-6-O-sulfotransferase 1 (GlcNAc6ST-1) and Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST-2), was previously shown to act on non-reducing GlcNAc residues (3). The enzyme is known to be involved in biosynthesis of L-selectin ligand sialyl 6-sulfo Lewis X (4) and therefore plays a role in lymphocyte homing (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.