B4GALT3 Antibody (OTI1G9) - Azide and BSA Free

Western Blot: B4GALT3 Antibody (OTI1G9) - Azide and BSA Free [NBP2-71386] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B4GALT3.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: OTI1G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

B4GALT3 Antibody (OTI1G9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse B4GALT3 Antibody (OTI1G9) - Azide and BSA Free (NBP2-46561) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 100-393 of human B4GALT3(NP_003770) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: B4GALT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 43.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for B4GALT3 Antibody (OTI1G9) - Azide and BSA Free

• b4Gal-T3
• beta-1,4-galactosyltransferase 3
• Beta-1,4-GalTase 3
• beta4Gal-T3
• beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 3
• EC 2.4.1.-
• UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3
• UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
• UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3

Background

Several oligosaccharide structures and protein glycoconjugate types are found in nature. Homologous glycosyltransferase (GT) gene families catalyze the formation of glycosidic linkages. The beta-1,3 galactosyltransferase(beta3GalT) gene family encodes a set of type II transmembrane glycoproteins that are catalytically diverse and use different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine ) to catalyze the addition of an activated monosaccharide to a terminal lactose. The protein coding sequences for beta-1,3-Gal-T genes comprise a single exon and are distantly related to the Drosophila Brainiac gene. The beta-1,4-galactosyltransferase (beta4GalT) gene family encodes type II membrane-bound glycoproteins that show exclusive specificity for the donor substrate, UDP-galactose. beta-1,4Gal-T genes transfer galactose in a beta-1,4 linkage to similar acceptor sugars; each gene has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B4GALT3