AP3B2 Antibody

Western Blot: AP3B2 Antibody [NB100-74370] - Figure 1 illustrates Western blot detection of beta-NAP at ~125 kDa using NB100-74370. A ~50 kDa band is also seen in rat and mouse brain tissue, representing the medium chain homolog (p47A) of AP3.

Product Discontinued

AP3B2 Antibody Summary

• Immunogen: Syntehtic peptides corresponding to residues L(260) L E E N (A/P) E K A F Y G S E E(274) of human and mouse beta NAP.
• Specificity: beta NAP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AP3B2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Application Notes: In WB: Detects an approx. 119 - 130 kDa protein representing beta NAP from brain samples.
• Control:

  CTNNBL1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AP3B2 Antibody

• Adapter-related protein complex 3 subunit beta-2
• Adaptor protein complex AP-3 subunit beta-2
• adaptor-related protein complex 3, beta 2 subunit
• beta-3B-adaptin
• Clathrin assembly protein complex 3 beta-2 large chain
• DKFZp686D17136
• NAPTBAP-3 complex subunit beta-2
• Neuronal adaptin-like protein, beta-subunit
• Neuron-specific vesicle coat protein beta-NAP

Background

Beta NAP is closely related to the beta-adaptin and beta-COP coat proteins. Beta NAP is a nonclathrin-associated phosphoprotein expressed exclusively in neurons, from E12 through adulthood. Beta NAP is present in the neurnal soma and nerve terminal as soluble and membrane-bound pools and is associated with a discrete set of nerve-terminal vesicles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AP3B2
• Entrez:
  • 8120(Human)
  • 11775(Mouse)
  • 308777(Rat)
• Uniprot:
  • Q13367(Human)
  • Q9JME5(Mouse)