Alpha Amylase Antibody [Biotin]

Images

 
Western Blot: Alpha Amylase Antibody [Biotin] [NBP1-77711] - Lane 1: Alpha Amylase solution. Load: 1/200 dilution of Alpha Amylase solution. Primary antibody: Alpha Amylase antibody at 1:1,000 overnight at 4C. Secondary ...read more

Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Alpha Amylase Antibody [Biotin] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Alpha Amylase [Bacillus amyloliquefaciens]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:5000
  • ELISA 1:5000 - 1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of a-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:4,000 - 1:20,000 of the reconstitution concentration is suggested for this product.
Readout System

Reactivity Notes

Reactive with Alpha Amylase from Bacillus amyloliquefaciens. Cross-reactivity against Alpha Amylase from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Delipidation and Defibrination
Reconstitution Instructions
Reconstitute with 100 ul deionized water

Background

Salivary Amylase is a digestive enzyme secreted by salivary glands, it consists of a single polypeptide chain with a molecular weight of 56kDa (family B) or 62kDa (family A), the difference in molecular weight being due to the presence or absence of a carbohydrate moiety. The total serum concentration of amylase is about 120g/L of which about 50% is salivary amylase. Although salivary amylase and pancreatic amylase share 95% amino acid sequence homology, they differ in molecular size, isoelectricpoint and antigenic properties.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alpha Amylase Antibody (NBP1-77711) (0)

There are no publications for Alpha Amylase Antibody (NBP1-77711).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Alpha Amylase Antibody (NBP1-77711) (0)

There are no reviews for Alpha Amylase Antibody (NBP1-77711). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Alpha Amylase Antibody (NBP1-77711) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NBP1-77711
HRP NB100-2015

Additional Alpha Amylase Products

Array NBP1-77711

Blogs on Alpha Amylase

There are no specific blogs for Alpha Amylase, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Alpha Amylase Antibody [Biotin] and receive a gift card or discount.