Alpha Amylase Antibody

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Western Blot: Alpha Amylase Antibody [NBP1-77792] - Analysis using the Biotin conjugate of NBP1-77792. Detection of Lane 1: Alpha Amylase solution. Load: 1/200 dilution of Alpha Amylase solution. Primary antibody: Alpha ...read more

Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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Alpha Amylase Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Alpha Amylase [Bacillus amyloliquefaciens]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of a-Amylase [Bacillus amyloliquefaciens] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:20,000 - 1:100,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Reactive with Alpha Amylase from Bacillus amyloliquefaciens. Cross-reactivity against Alpha Amylase from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Background

Salivary Amylase is a digestive enzyme secreted by salivary glands, it consists of a single polypeptide chain with a molecular weight of 56kDa (family B) or 62kDa (family A), the difference in molecular weight being due to the presence or absence of a carbohydrate moiety. The total serum concentration of amylase is about 120g/L of which about 50% is salivary amylase. Although salivary amylase and pancreatic amylase share 95% amino acid sequence homology, they differ in molecular size, isoelectricpoint and antigenic properties.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NBP1-77711
HRP NB100-2015

Additional Alpha Amylase Products

Array NBP1-77792

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