Alpha Amylase Antibody


Western Blot: Alpha Amylase Antibody [NBP1-77792] - Analysis using the Biotin conjugate of NBP1-77792. Detection of Lane 1: Alpha Amylase solution. Load: 1/200 dilution of Alpha Amylase solution. Primary antibody: Alpha more

Product Details

Reactivity BaSpecies Glossary
Applications WB, ELISA, IP
1 mg/ml

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Alpha Amylase Antibody Summary

This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Alpha Amylase [Bacillus amyloliquefaciens]
Ion exchange chromatography
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  • Western Blot 1:500-1:5000
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of a-Amylase [Bacillus amyloliquefaciens] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:20,000 - 1:100,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Reactive with Alpha Amylase from Bacillus amyloliquefaciens. Cross-reactivity against Alpha Amylase from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
1 mg/ml
Ion exchange chromatography


Salivary Amylase is a digestive enzyme secreted by salivary glands, it consists of a single polypeptide chain with a molecular weight of 56kDa (family B) or 62kDa (family A), the difference in molecular weight being due to the presence or absence of a carbohydrate moiety. The total serum concentration of amylase is about 120g/L of which about 50% is salivary amylase. Although salivary amylase and pancreatic amylase share 95% amino acid sequence homology, they differ in molecular size, isoelectricpoint and antigenic properties.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin NBP1-77711
HRP NB100-2015

Additional Alpha Amylase Products

Array NBP1-77792

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