3-OHKYN Antibody (P3UI) - BSA Free

Western Blot: 3-OHKYN Antibody (P3UI) [NB100-597] - Detection of 3'OHKYN in modified BSA using NB 100-597. 10 second ECL exposure. Lane 1: BSA (-) Lane 2: BSA (+).

Immunohistochemistry-Paraffin: 3-OHKYN Antibody (P3UI) [NB100-597] - 3-OHKYN was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human 3-OHKYN (P3UI) Monoclonal Antibody (Catalog # NB100-597) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in neurons.

• Reactivity: Hu, All
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: P3UI
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 2.6 mg/ml

3-OHKYN Antibody (P3UI) - BSA Free Summary

• Description: Novus Biologicals Mouse 3-OHKYN Antibody (P3UI) - BSA Free (NB100-597) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-3-OHKYN Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: 3-OHKYN modified KLH
• Localization: Cytoplasmic
• Specificity: This is specific for 3-hydroxykynureinine (3OH-Kyn) modifications on proteins. It is likely to react with 3OH-Kyn modification on lysine, histidine and cysteine residues on proteins. It does not react with enzymes involved in the degradation of tryptopha
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA reported in scientific literature
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin reported in scientific literature
  • Western Blot 2 ug/ml
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 2.6 mg/ml
• Purity: Protein G purified

Alternate Names for 3-OHKYN Antibody (P3UI) - BSA Free

• 3039Hydroxykynurenine
• 3-hydroxykynuenine modification

Background

3-OHKYN (3-Hydroxykynurenine) is tryptophan's toxic metabolite which is capable of inducing oxidative damage as well as cell death consequently contributing to different neurological and psychiatric disorders. Tryptophan is either transaminated to kynurenate (an endogenous glutamate receptor antagonist), or hydroxylated to 3-OHKYN which is degraded to excitotoxic N-methyl-D-aspartate (NMDA) agonist, quinolinate. Under physiological conditions, kynurenine pathway is activated to form metabolic precursor NAD+; however, under inflammatory, oxidative or neurotoxic attack, there is an increased activation of indoleamine dioxygenase (IDO), kynurenine monooxygenase (KMO) and tryptophan dioxygenase (TDO), yielding the formation of toxic metabolites such as 3-OHKYN and quinolinic acid. Once 3-OHKYN crosses neuronal cell membranes through neutral amino acid transporter (NAT), it can follow two routes: (i) may act as ROS scavenger/antioxidant via direct scavenging or by triggering integral antioxidant responses evoked by transcription signaling pathways, such as the Nrf2/Keap1/ARE axis (ii) 3-OHKYN may undergoes autooxidation yielding highly reactive molecules, such as o-aminoquinone, which in turn causes exacerbated oxidative damage, activation of deadly cascades, and massive cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 3-OHKYN Antibody (NB100-597)(2)

Publications using NB100-597

• Deepak Prasad Gupta, Sung Hee Park, Hyun-Jeong Yang, Kyoungho Suk, Gyun Jee Song Neuroprotective and Anti—Neuroinflammatory Effects of a Poisonous Plant Croton tiglium Linn. Extract Toxins 2020-04-17 [PMID: 32316571]
• Staniszewska, MM, Nagaraj, RH. 3-hydroxykynurenine-mediated modification of human lens proteins: structure determination of a major modification using a monoclonal antibody. J Biol Chem. 280(23):22154-64. Epub 2005 Apr 6. 2005-06-10 [PMID: 15817458] (ELISA, WB, IHC-P, ICC/IF, Human)