3-OHKYN Antibody (P3UI)

Images

 
Western Blot: 3-OHKYN Antibody (P3UI) [NB100-597] - Detection of 3'OHKYN in modified BSA using NB 100-597. 10 second ECL exposure. Lane 1: BSA (-) Lane 2: BSA (+).
Immunohistochemistry-Paraffin: 3-OHKYN Antibody (P3UI) [NB100-597] - 3-OHKYN was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human 3-OHKYN (P3UI) Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, AllSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
P3UI
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2.6 mg/ml

Order Details

3-OHKYN Antibody (P3UI) Summary

Immunogen
3-OHKYN modified KLH
Localization
Cytoplasmic
Specificity
This is specific for 3-hydroxykynureinine (3OH-Kyn) modifications on proteins. It is likely to react with 3OH-Kyn modification on lysine, histidine and cysteine residues on proteins. It does not react with enzymes involved in the degradation of tryptopha
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA reported in scientific literature
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin reported in scientific literature
  • Western Blot 2 ug/ml
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB100-597 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
2.6 mg/ml
Purity
Protein G purified

Alternate Names for 3-OHKYN Antibody (P3UI)

  • 3039Hydroxykynurenine
  • 3-hydroxykynuenine modification

Background

3-OHKYN (3-Hydroxykynurenine) is tryptophan's toxic metabolite which is capable of inducing oxidative damage as well as cell death consequently contributing to different neurological and psychiatric disorders. Tryptophan is either transaminated to kynurenate (an endogenous glutamate receptor antagonist), or hydroxylated to 3-OHKYN which is degraded to excitotoxic N-methyl-D-aspartate (NMDA) agonist, quinolinate. Under physiological conditions, kynurenine pathway is activated to form metabolic precursor NAD+; however, under inflammatory, oxidative or neurotoxic attack, there is an increased activation of indoleamine dioxygenase (IDO), kynurenine monooxygenase (KMO) and tryptophan dioxygenase (TDO), yielding the formation of toxic metabolites such as 3-OHKYN and quinolinic acid. Once 3-OHKYN crosses neuronal cell membranes through neutral amino acid transporter (NAT), it can follow two routes: (i) may act as ROS scavenger/antioxidant via direct scavenging or by triggering integral antioxidant responses evoked by transcription signaling pathways, such as the Nrf2/Keap1/ARE axis (ii) 3-OHKYN may undergoes autooxidation yielding highly reactive molecules, such as o-aminoquinone, which in turn causes exacerbated oxidative damage, activation of deadly cascades, and massive cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 3-OHKYN Antibody (NB100-597)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA, ICC/IF, IHC-P, WB.


Filter By Application
ELISA
(1)
ICC/IF
(1)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

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Product General Protocols

View specific protocols for 3-OHKYN Antibody (NB100-597): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional 3-OHKYN Products

Array NB100-597

Research Areas for 3-OHKYN Antibody (NB100-597)

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Blogs on 3-OHKYN

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