3-OHKYN Antibody (P3UI) [Allophycocyanin]

• Reactivity: Hu, All
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: P3UI
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

3-OHKYN Antibody (P3UI) [Allophycocyanin] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: 3-OHKYN modified KLH
• Localization: Cytoplasmic
• Specificity: This is specific for 3-hydroxykynureinine (3OH-Kyn) modifications on proteins. It is likely to react with 3OH-Kyn modification on lysine, histidine and cysteine residues on proteins. It does not react with enzymes involved in the degradation of tryptopha
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This 3-OHKYN antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~66 kDa. Immunohistochemistry-Paraffin, and ELISA were reported in scientific literature.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
  
  Recommended applications are based on validated applications from the unconjugated base product NB100-597. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, although there should be no species limitation to this antibody's reactivity.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for 3-OHKYN Antibody (P3UI) [Allophycocyanin]

• 3039Hydroxykynurenine
• 3-hydroxykynuenine modification

Background

3-hydroxykynurenine is a chromophore metabolite of tryptophan, which acts as UV protectant and an antioxidant in vertebrate lens. 3-OHKYN reacts with proteins to induce cross-linking and chromophore adducts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.