TNF-alpha Antibodies

1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Canine TNF-a (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507). The ND₅₀ is typically 0.4-1.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Canine TNF-a (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507). The ND₅₀ is typically 0.4-1.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Canine TNF-a (1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Canine TNF-a (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507). The ND₅₀ is typically 0.4-1.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1507-CT) was serially diluted and captured by Mouse Anti-Canine TNF‑ alpha Monoclonal Antibody (Catalog # MAB1507) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1507-CT) was serially diluted and captured by Mouse Anti-Canine TNF‑ alpha Monoclonal Antibody (Catalog # MAB1507) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood mononuclear cells (PBMCs) (A) treated with PMA and Ca2+ ionomycin overnight, or (B) resting, were stained with Human Anti-Human TNF- alpha (Research Grade Adalimumab Biosimilar) Monoclonal Antibody (Catalog # MAB9677) followed by Goat anti-Human APC-conjugated Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human TNF-a (Research Grade Adalimumab Biosimilar) Monoclonal Antibody (Catalog # MAB9677). The ND₅₀ is typically 2-12 ng/mL in the presence of the metabolic inhibitor actinomycin D." class="big_lightbox"> 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human TNF-a (Research Grade Adalimumab Biosimilar) Monoclonal Antibody (Catalog # MAB9677). The ND₅₀ is typically 2-12 ng/mL in the presence of the metabolic inhibitor actinomycin D." class="big_thumb" />

1814-ET) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Equine TNF-a (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1814). The ND₅₀ is typically 0.01-0.03 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 1814-ET) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Equine TNF-a (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1814). The ND₅₀ is typically 0.01-0.03 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Equine TNF-a (Catalog # 1814-ET) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Equine TNF-a (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1814). The ND₅₀ is typically 0.01-0.03 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1814-ET) was serially diluted and captured by Goat Anti-Equine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1814) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine TNF‑ alpha Biotinylated Antigen Affinity-purified Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 1814-ET) was serially diluted and captured by Goat Anti-Equine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1814) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine TNF‑ alpha Biotinylated Antigen Affinity-purified Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

HAF018). Specific bands were detected for TNF-a at approximately 13 and 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). Specific bands were detected for TNF-a at approximately 13 and 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- a. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB2102) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for TNF-a at approximately 13 and 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

690-PT) induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF-a (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine TNF-a Monoclonal Antibody (Catalog # MAB6901). The ND₅₀ is typically 0.015-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 690-PT) induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF-a (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine TNF-a Monoclonal Antibody (Catalog # MAB6901). The ND₅₀ is typically 0.015-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Porcine TNF-a (Catalog # 690-PT) induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF-a (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine TNF-a Monoclonal Antibody (Catalog # MAB6901). The ND₅₀ is typically 0.015-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />