TNF-alpha Antibodies

1011-CR) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Cotton Rat TNF-a (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat TNF-a Monoclonal Antibody (Catalog # MAB10111). The ND₅₀ is typically 0.04-0.2 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 1011-CR) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Cotton Rat TNF-a (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat TNF-a Monoclonal Antibody (Catalog # MAB10111). The ND₅₀ is typically 0.04-0.2 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Cotton Rat TNF-a (Catalog # 1011-CR) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Cotton Rat TNF-a (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat TNF-a Monoclonal Antibody (Catalog # MAB10111). The ND₅₀ is typically 0.04-0.2 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

1011-CR) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002) . Cytotoxicity elicited by Recombinant Cotton Rat TNF-a (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat TNF-a Monoclonal Antibody (Catalog # MAB1011). The ND₅₀ is typically 0.05- 0.3 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 1011-CR) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002) . Cytotoxicity elicited by Recombinant Cotton Rat TNF-a (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat TNF-a Monoclonal Antibody (Catalog # MAB1011). The ND₅₀ is typically 0.05- 0.3 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Cotton Rat TNF-a (Catalog # 1011-CR) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002) . Cytotoxicity elicited by Recombinant Cotton Rat TNF-a (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat TNF-a Monoclonal Antibody (Catalog # MAB1011). The ND₅₀ is typically 0.05- 0.3 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />

1070-RM) was serially diluted and captured by Rat Anti-Rhesus Macaque TNF‑ alpha Monoclonal Antibody (Catalog # MAB10702) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rhesus Macaque TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1070) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1070-RM) was serially diluted and captured by Rat Anti-Rhesus Macaque TNF‑ alpha Monoclonal Antibody (Catalog # MAB10702) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rhesus Macaque TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1070) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Rhesus Macaque TNF‑ alpha (Catalog # 1070-RM) was serially diluted and captured by Rat Anti-Rhesus Macaque TNF‑ alpha Monoclonal Antibody (Catalog # MAB10702) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rhesus Macaque TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1070) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1070-RM) was serially diluted and captured by Rat Anti-Rhesus Macaque TNF‑ alpha Monoclonal Antibody (Catalog # MAB10702) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rhesus Macaque TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1070) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1070-RM) was serially diluted and captured by Rat Anti-Rhesus Macaque TNF‑ alpha Monoclonal Antibody (Catalog # MAB10702) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rhesus Macaque TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1070) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Rhesus Macaque TNF‑ alpha (Catalog # 1070-RM) was serially diluted and captured by Rat Anti-Rhesus Macaque TNF‑ alpha Monoclonal Antibody (Catalog # MAB10702) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rhesus Macaque TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1070) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

5035-TG) was serially diluted and captured by Mouse Anti-Guinea Pig TNF‑ alpha Monoclonal Antibody (Catalog # MAB5035) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Guinea Pig TNF‑ alpha Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 5035-TG) was serially diluted and captured by Mouse Anti-Guinea Pig TNF‑ alpha Monoclonal Antibody (Catalog # MAB5035) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Guinea Pig TNF‑ alpha Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Guinea Pig TNF‑ alpha (Catalog # 5035-TG) was serially diluted and captured by Mouse Anti-Guinea Pig TNF‑ alpha Monoclonal Antibody (Catalog # MAB5035) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Guinea Pig TNF‑ alpha Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

MAB002) followed by Anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B) and Mouse anti-Human CD3 APC-conjugated Monoclonal Antibody (FAB100A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." class="big_lightbox"> MAB002) followed by Anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B) and Mouse anti-Human CD3 APC-conjugated Monoclonal Antibody (FAB100A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." alt="Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Ca2+ionomycin for 3.5 hours were stained with (A) Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # MAB10564) or (B) isotype control antibody (MAB002) followed by Anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B) and Mouse anti-Human CD3 APC-conjugated Monoclonal Antibody (FAB100A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." class="big_thumb" />