N6-methyladenosine (m6A) Antibodies
We offer N6-methyladenosine (m6A) Antibodies for use in common research applications: Dot Blot, Immunoprecipitation, Western Blot. Each N6-methyladenosine (m6A) Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our N6-methyladenosine (m6A) Antibodies can be used in a variety of model species: Human, Mouse, Yeast. Use the list below to choose the N6-methyladenosine (m6A) Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our N6-methyladenosine (m6A) monoclonal antibodies.
Alternate Names for N6-methyladenosine (m6A) Antibodies
anti-N6-methyladenosine (m6A) antibody
11 Results for "n6-methyladenosine-(m6a)" in Primary Antibodies
![Western Blot: N6-methyladenosine (m6A) Antibody (17-3-4-1) [NBP2-50525]](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-17-3-4-1-Western-Blot-NBP2-50525-img0004.jpg "Western Blot: N6-methyladenosine (m6A) Antibody (17-3-4-1) [NBP2-50525]")
![Western Blot: N6-methyladenosine (m6A) Antibody (17-3-4-1) [NBP2-50525]](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-17-3-4-1-Western-Blot-NBP2-50525-img0001.jpg)
![Western Blot: N6-methyladenosine (m6A) Antibody (17-3-4-1) [NBP2-50525]](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-17-3-4-1-Western-Blot-NBP2-50525-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: N6-methyladenosine (m6A) Antibody [NBP3-05657] - U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37C for the indicated time, and stained for m6A Polyclonal N6-methyladenosine (m6A)antibody, DAPI, 4', 6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel, peaking at 2 min after irradiation and diminishing over the following 8 min.](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05657-img0004.jpg "Immunocytochemistry/Immunofluorescence: N6-methyladenosine (m6A) Antibody [NBP3-05657] - U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37C for the indicated time, and stained for m6A Polyclonal N6-methyladenosine (m6A)antibody, DAPI, 4', 6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel, peaking at 2 min after irradiation and diminishing over the following 8 min.")
![N/A: N6-methyladenosine (m6A) Antibody [NBP3-05657] - N6-methyladenosine (m6A) antibody is tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).Oligomer 1 - N6-methyladenosine (m6A-CUGGUAACGAAUGGCU-G-Biotin)Oligomer 2 - unmodified adenosine (ACUGGUAACGAAUGGCU-G-Biotin)Oligomer 3 - unmodified adenosine (AAAAAAAAAAAAAAAAA-Biotin)](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-N-A-NBP3-05657-img0001.jpg)
![Dot Blot: N6-methyladenosine (m6A) Antibody [NBP3-05657] - N6-methyladenosine (m6A) antibody) are tested in Dot Blot against total RNA of Arabidopsis.](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-Dot-Blot-NBP3-05657-img0002.jpg)
![Dot Blot: N6-methyladenosine (m6A) Antibody [NBP3-05657] - N6-methyladenosine (m6A) antibody are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.Oligomer 8 - ATAACTGG-m6A-CCGAATGGOligomer 9 - ATAACTGGACCGAATGGOligomer 10 - AAAAAAAAAAAAAAAA-biotin.](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-Dot-Blot-NBP3-05657-img0003.jpg)
![Immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-05657] - Immunoprecipitation analysis of 25ug total RNA extracts of Arabidopsis (25ug total RNA extracts of Arabidopsis was diluted to 200 ul IP buffer system) using 2ul N6-methyladenosine (m6A) antibody.](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-Immunoprecipitation-NBP3-05657-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: N6-methyladenosine (m6A) Antibody (7J1S3) [NBP3-33361] - U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 C for the indicated time,immunofluorescence analysis was performed by N6-methyladenosine (m6A) Rabbit mAb,DAPI, 4',6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel,peaking at 2 min after irradiation and diminishing over the following 8 min.](http://images.novusbio.com/fullsize/nbp3-33361_rabbit-n6-methyladenosine-m6a-mab-7j1s3-912202415143659.jpg "Immunocytochemistry/ Immunofluorescence: N6-methyladenosine (m6A) Antibody (7J1S3) [NBP3-33361] - U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 C for the indicated time,immunofluorescence analysis was performed by N6-methyladenosine (m6A) Rabbit mAb,DAPI, 4',6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel,peaking at 2 min after irradiation and diminishing over the following 8 min.")
![Dot Blot: N6-methyladenosine (m6A) Antibody (7J1S3) [NBP3-33361] - Dot-blot analysis of all sorts of peptides using N6-methyladenosine (m6A) antibody at dilution.](http://images.novusbio.com/fullsize/nbp3-33361_rabbit-n6-methyladenosine-m6a-mab-7j1s3-912202415171183.jpg)
![Dot Blot: N6-methyladenosine (m6A) Antibody (7J1S3) [NBP3-33361] - The N6-methyladenosine / m6A Rabbit mAb are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.Oligomer 8 - ATAACTGG-m6A-CCGAATGGOligomer 9 - ATAACTGGACCGAATGGOligomer 10 - AAAAAAAAAAAAAAAA-biotin.](http://images.novusbio.com/fullsize/nbp3-33361_rabbit-n6-methyladenosine-m6a-mab-7j1s3-10122024140432.jpg)
![Dot Blot: N6-methyladenosine (m6A) Antibody (7J1S3) [NBP3-33361] - The N6-methyladenosine (m6A) Rabbit mAb (4ug,A19841) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).Oligomer 4 - N6-methyladenosine (m6A-UAACUGGACCGAAUGG-Biotin)Oligomer 5 - N6-methyladenosine Oligomer 6 - unmodified adenosine](http://images.novusbio.com/fullsize/nbp3-33361_rabbit-n6-methyladenosine-m6a-mab-7j1s3-52202511365124.jpg)
![Dot Blot: N6-methyladenosine (m6A) Antibody (7J1S3) [N6-methyladenosine (m6A)] - The N6-methyladenosine (m6A) Rabbit mAb (4ug,A19841) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).Oligomer 4 - N6-methyladenosine (m6A-UAACUGGACCGAAUGG-Biotin)Oligomer 5 - N6-methyladenosine Oligomer 6 - unmodified adenosine](http://images.novusbio.com/fullsize/nbp3-33361_rabbit-n6-methyladenosine-m6a-mab-7j1s3-52202517352737.jpg)
![Dot Blot: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 4. Dot blot analysis using the antibody directed against N6-methyladenosine (m6A) To demonstrate the specificity of the antibody against N6-methyladenosine (m6A) , a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligos were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-Dot-Blot-NBP3-18690-img0001.jpg "Dot Blot: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 4. Dot blot analysis using the antibody directed against N6-methyladenosine (m6A) To demonstrate the specificity of the antibody against N6-methyladenosine (m6A) , a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligos were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.")
![RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 1. RNA immunoprecipitation using the antibody directed against N6-methyladenosine (m6A) RNA Immunoprecipitation was performed on 40 ug HeLa total RNA spiked with 0.5 ug of an in vitro prepared transcript containing N6-methyladenosine (m6A) nucleotides, using 2 ug of the N6-methyladenosine (m6A) antibody . An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-RNA-immunoprecipitation-NBP3-18690-img0002.jpg)
![RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 3. RIP-seq results obtained with the antibody directed against N6-methyladenosine (m6A) RIP was performed with 2 ug of the antibody against N6-methyladenosine (m6A) . The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-RNA-immunoprecipitation-NBP3-18690-img0004.jpg)
![RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 2. RNA immunoprecipitation using the antibody directed against N6-methyladenosine (m6A) RNA Immunoprecipitation was performed on 40 ug HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 ug of antibody per RIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3 UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-RNA-immunoprecipitation-NBP3-18690-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: N6-methyladenosine (m6A) Antibody (6S5W2) [NBP3-35057] - Immunofluorescence analysis of U-2 OS treated with UV(30s)+Brdu(0min) and U-2 OS treated with UV(30s)+Brdu(10min) cells using N6-methyladenosine (m6A) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35057_rabbit-n6-methyladenosine-m6a-mab-6s5w2-1012202411343368.jpg "Immunocytochemistry/ Immunofluorescence: N6-methyladenosine (m6A) Antibody (6S5W2) [NBP3-35057] - Immunofluorescence analysis of U-2 OS treated with UV(30s)+Brdu(0min) and U-2 OS treated with UV(30s)+Brdu(10min) cells using N6-methyladenosine (m6A) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Dot Blot: N6-methyladenosine (m6A) Antibody (6S5W2) [NBP3-35057] - The m6A rabbit monoclonal antibody are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.Oligomer 8 - ATAACTGG-m6A-CCGAATGGOligomer 9 - ATAACTGGACCGAATGGOligomer 10 - AAAAAAAAAAAAAAAA-biotin.](http://images.novusbio.com/fullsize/nbp3-35057_rabbit-n6-methyladenosine-m6a-mab-6s5w2-10122024140444.jpg)
![RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody (6S5W2) [NBP3-35057] - RNA Immunoprecipitation was performed on 100 µg mouse liver total RNA ,using 5 µg of the N6-methyladenosine / m6A Rabbit mAb. An equal amount of IgG was used as negative control. The immunoprecipitated RNA was verified by using HS6ST1 as PCR primer of qRT-PCR . The picture shows the relative enrichment multiple of HS6ST1 site.](http://images.novusbio.com/fullsize/nbp3-35057_rabbit-n6-methyladenosine-m6a-mab-6s5w2-10122024140427.jpg)
![Immunoprecipitation: N6-methyladenosine (m6A) Antibody (C.15200082) [NBP2-59171] - Immunoprecipitation was performed on two radiolabelled synthetic DNA oligos, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the monoclonal m6A antibody. The immunoprecipitated fraction is shown in lane 3. Lane 2 shows the non bound fraction, whereas the input is shown in lane 1.](http://images.novusbio.com/fullsize/N6-Methyladenosine-Antibody-C-15200082-Dot-Blot-NBP2-59171-img0001.jpg "Immunoprecipitation: N6-methyladenosine (m6A) Antibody (C.15200082) [NBP2-59171] - Immunoprecipitation was performed on two radiolabelled synthetic DNA oligos, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the monoclonal m6A antibody. The immunoprecipitated fraction is shown in lane 3. Lane 2 shows the non bound fraction, whereas the input is shown in lane 1.")
![Dot Blot: N6-methyladenosine (m6A) Antibody (C.15200082) [NBP2-59171] - Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligos were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10% skimmed milk and 1% BSA. Figure shows a high specificity of the antibody for the modified oligonucleotide.](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-C-15200082-Dot-Blot-NBP2-59171-img0004.jpg)
![Immunoprecipitation: N6-methyladenosine (m6A) Antibody (C.15200082) [NBP2-59171] - RNA Immunoprecipitation was performed on 40 ug HeLa total RNA spiked with 0.5 ug of an in vitro prepared transcript containing 6mA nucleotides, using 2 ug of the monoclonal m6A antibody. An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure shows the recovery, expressed as % input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-C-15200082-Immunoprecipitation-NBP2-59171-img0005.jpg)
![Immunoprecipitation: N6-methyladenosine (m6A) Antibody (RM362) [NBP2-77425] - MeRIP was performed using M6A and control RNA from NEB (E1610). Antibodies (NBP2-77425 and NBP2-77399) were mixed for a short time, with Protein A beads before the addition of m6A labelled and non- labelled RNA (in equal amounts). Captured RNA was then washed, eluted and run through RT-PCR. Resulting cDNA was then analyzed using Realtime PCR with specific probes to m6A labelled and un-labelled sequences.](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-RM362-Immunoprecipitation-NBP2-77425-img0002.jpg "Immunoprecipitation: N6-methyladenosine (m6A) Antibody (RM362) [NBP2-77425] - MeRIP was performed using M6A and control RNA from NEB (E1610). Antibodies (NBP2-77425 and NBP2-77399) were mixed for a short time, with Protein A beads before the addition of m6A labelled and non- labelled RNA (in equal amounts). Captured RNA was then washed, eluted and run through RT-PCR. Resulting cDNA was then analyzed using Realtime PCR with specific probes to m6A labelled and un-labelled sequences.")
![Dot Blot: N6-methyladenosine (m6A) Antibody (RM362) [NBP2-77425] - Dot blot of Lambda DNA without or with m6A, using NBP2-77425. The membrane was pre-spotted with lambda DNA. m6A-free DNA was isolated from bacteriophage lambda grown in an E. coli host-deficient in adenine methylase (dam-).](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-RM362-Dot-Blot-NBP2-77425-img0001.jpg)
![ELISA: N6-methyladenosine (m6A) Antibody (1G1) [NBP3-32554] - Competitive ELISA analysis of N6-methyladenosine (m6A) was performed by coating wells of a 96-well plate with 50 µl per well of m6A -BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1% BSA blocking buffer, and incubated with 100 µl per well of m6A monoclonal antibody at concentration of 1 µg/mL with serial diluted m6A and its analogs starting from a concentration of 10,000 ng/ml to 78ng/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32554_rabbit-n6-methyladenosine-m6a-mab-1g1-27820241141612.jpg "ELISA: N6-methyladenosine (m6A) Antibody (1G1) [NBP3-32554] - Competitive ELISA analysis of N6-methyladenosine (m6A) was performed by coating wells of a 96-well plate with 50 µl per well of m6A -BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1% BSA blocking buffer, and incubated with 100 µl per well of m6A monoclonal antibody at concentration of 1 µg/mL with serial diluted m6A and its analogs starting from a concentration of 10,000 ng/ml to 78ng/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Dot Blot: N6-methyladenosine (m6A) Antibody (1G1) [NBP3-32554] - Dot blot analysis of N6-methyladenosine (m6A) using NBP3-32554. Loading: total RNA extracted from HeLa Blocking Buffer: 5% NFDM/TBST; Incubation time and temperature: 1 hours at room temperature; Primary Antibody: NBP3-32554 Dilution: 2 ug/ml, 1 ug/ml, 0.5 ug/ml, 0.25 ug/ml, 0.125 ug/ml, 0.0625 ug/ml Dilution Buffer: Primary Antibody Dilution Buffer; Incubation Time and Temperature: Overnight at 4C ; Secondary Antibody: Goat anti-Rabbit IgG-HRP antibody; Dilution: 1:300,000 Dilution Buffer: 5% NFDM/TBST; Incubation Time and Temperature: 30 minutes at room temperature; Exposure time: 60/120 seconds.](http://images.novusbio.com/fullsize/nbp3-32554_rabbit-n6-methyladenosine-m6a-mab-1g1-28820241459251.jpg)
![Dot Blot: N6-methyladenosine (m6A) Antibody (7N6C7) [NBP3-33194] - The m6A mouse monoclonal antibody are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine (100ng for each oligomer).Oligomer 8 - N6-methyladenosineOligomer 9 - unmodified adenosineOligomer 10 - unmodified adenosine](http://images.novusbio.com/fullsize/nbp3-33194_mouse-n6-methyladenosine-m6a-mab-7n6c7-10122024140420.jpg "Dot Blot: N6-methyladenosine (m6A) Antibody (7N6C7) [NBP3-33194] - The m6A mouse monoclonal antibody are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine (100ng for each oligomer).Oligomer 8 - N6-methyladenosineOligomer 9 - unmodified adenosineOligomer 10 - unmodified adenosine")