N6-methyladenosine (m6A) Antibody (C.15200082) - BSA Free

Immunoprecipitation: N6-methyladenosine (m6A) Antibody (C.15200082) [NBP2-59171] - Immunoprecipitation was performed on two radiolabelled synthetic DNA oligos, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the monoclonal m6A antibody. The immunoprecipitated fraction is shown in lane 3. Lane 2 shows the non bound fraction, whereas the input is shown in lane 1.

Dot Blot: N6-methyladenosine (m6A) Antibody (C.15200082) [NBP2-59171] - Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligos were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10% skimmed milk and 1% BSA. Figure shows a high specificity of the antibody for the modified oligonucleotide.

Immunoprecipitation: N6-methyladenosine (m6A) Antibody (C.15200082) [NBP2-59171] - RNA Immunoprecipitation was performed on 40 ug HeLa total RNA spiked with 0.5 ug of an in vitro prepared transcript containing 6mA nucleotides, using 2 ug of the monoclonal m6A antibody. An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure shows the recovery, expressed as % input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

• Reactivity: Hu, Mu
• Applications: DB, IP
• Clone: C.15200082
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

N6-methyladenosine (m6A) Antibody (C.15200082) - BSA Free Summary

• Description: Novus Biologicals Mouse N6-methyladenosine (m6A) Antibody (C.15200082) - BSA Free (NBP2-59171) is a monoclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The exact immunogen is propietary information.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:500
  • Immunoprecipitation 1 - 10 ug/IP

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide and 0.05% ProClin 300
• Purity: Protein A purified

Alternate Names for N6-methyladenosine (m6A) Antibody (C.15200082) - BSA Free

• (-)-6-(Methylamino)purine riboside
• N(6)-Methyladenosine
• N-Methyladenosine

Background

N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA's, rRNA's, snRNA's and in long non-coding RNA's. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3' UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it's physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.