![ELISA: N6-methyladenosine (m6A) Antibody (1G1) [NBP3-32554] - Competitive ELISA analysis of N6-methyladenosine (m6A) was performed by coating wells of a 96-well plate with 50 µl per well of m6A -BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1% BSA blocking buffer, and incubated with 100 µl per well of m6A monoclonal antibody at concentration of 1 µg/mL with serial diluted m6A and its analogs starting from a concentration of 10,000 ng/ml to 78ng/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32554_rabbit-n6-methyladenosine-m6a-mab-1g1-27820241141612.jpg "ELISA: N6-methyladenosine (m6A) Antibody (1G1) [NBP3-32554] - Competitive ELISA analysis of N6-methyladenosine (m6A) was performed by coating wells of a 96-well plate with 50 µl per well of m6A -BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1% BSA blocking buffer, and incubated with 100 µl per well of m6A monoclonal antibody at concentration of 1 µg/mL with serial diluted m6A and its analogs starting from a concentration of 10,000 ng/ml to 78ng/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
| Reactivity | All-NASpecies Glossary |
| Applications | DB, ELISA |
| Clone | 1G1 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit N6-methyladenosine (m6A) Antibody (1G1) (NBP3-32554) is a recombinant monoclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | N6-methyladenosine (m6A) Small Molecule conjugated to OVA. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Dot Blot: N6-methyladenosine (m6A) Antibody (1G1) [NBP3-32554] - Dot blot analysis of N6-methyladenosine (m6A) using NBP3-32554. Loading: total RNA extracted from HeLa Blocking Buffer: 5% NFDM/TBST; Incubation time and temperature: 1 hours at room temperature; Primary Antibody: NBP3-32554 Dilution: 2 ug/ml, 1 ug/ml, 0.5 ug/ml, 0.25 ug/ml, 0.125 ug/ml, 0.0625 ug/ml Dilution Buffer: Primary Antibody Dilution Buffer; Incubation Time and Temperature: Overnight at 4C ; Secondary Antibody: Goat anti-Rabbit IgG-HRP antibody; Dilution: 1:300,000 Dilution Buffer: 5% NFDM/TBST; Incubation Time and Temperature: 30 minutes at room temperature; Exposure time: 60/120 seconds.](http://images.novusbio.com/fullsize/nbp3-32554_rabbit-n6-methyladenosine-m6a-mab-1g1-28820241459251.jpg "Dot Blot: N6-methyladenosine (m6A) Antibody (1G1) [NBP3-32554] - Dot blot analysis of N6-methyladenosine (m6A) using NBP3-32554. Loading: total RNA extracted from HeLa Blocking Buffer: 5% NFDM/TBST; Incubation time and temperature: 1 hours at room temperature; Primary Antibody: NBP3-32554 Dilution: 2 ug/ml, 1 ug/ml, 0.5 ug/ml, 0.25 ug/ml, 0.125 ug/ml, 0.0625 ug/ml Dilution Buffer: Primary Antibody Dilution Buffer; Incubation Time and Temperature: Overnight at 4C ; Secondary Antibody: Goat anti-Rabbit IgG-HRP antibody; Dilution: 1:300,000 Dilution Buffer: 5% NFDM/TBST; Incubation Time and Temperature: 30 minutes at room temperature; Exposure time: 60/120 seconds.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")