![Immunoprecipitation: N6-methyladenosine (m6A) Antibody (RM362) [NBP2-77425] - MeRIP was performed using M6A and control RNA from NEB (E1610). Antibodies (NBP2-77425 and NBP2-77399) were mixed for a short time, with Protein A beads before the addition of m6A labelled and non- labelled RNA (in equal amounts). Captured RNA was then washed, eluted and run through RT-PCR. Resulting cDNA was then analyzed using Realtime PCR with specific probes to m6A labelled and un-labelled sequences.](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-RM362-Immunoprecipitation-NBP2-77425-img0002.jpg "Immunoprecipitation: N6-methyladenosine (m6A) Antibody (RM362) [NBP2-77425] - MeRIP was performed using M6A and control RNA from NEB (E1610). Antibodies (NBP2-77425 and NBP2-77399) were mixed for a short time, with Protein A beads before the addition of m6A labelled and non- labelled RNA (in equal amounts). Captured RNA was then washed, eluted and run through RT-PCR. Resulting cDNA was then analyzed using Realtime PCR with specific probes to m6A labelled and un-labelled sequences.")
| Reactivity | HuSpecies Glossary |
| Applications | DB, ELISA, IP |
| Clone | RM362 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit N6-methyladenosine (m6A) Antibody (RM362) (NBP2-77425) is a recombinant monoclonal antibody validated for use in ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | BSA-conjugated N6-methyladenosine (m6A) |
| Specificity | This antibody reacts to N6-Methyladenosine (m6A) in both RNA and DNA. No cross reactivity with non-methylated adenosine. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Can be used in Methylated RNA Immunoprecipitation (recommended dilution 0.2 - 5 ug/ml) |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 50% Glycerol/PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Dot Blot: N6-methyladenosine (m6A) Antibody (RM362) [NBP2-77425] - Dot blot of Lambda DNA without or with m6A, using NBP2-77425. The membrane was pre-spotted with lambda DNA. m6A-free DNA was isolated from bacteriophage lambda grown in an E. coli host-deficient in adenine methylase (dam-).](http://images.novusbio.com/fullsize/nbp2-77425_rabbit-monoclonal-n6-methyladenosine-m6a-antibody-rm362-dot-blot-18920252344476.png "Dot Blot: N6-methyladenosine (m6A) Antibody (RM362) [NBP2-77425] - Dot blot of Lambda DNA without or with m6A, using NBP2-77425. The membrane was pre-spotted with lambda DNA. m6A-free DNA was isolated from bacteriophage lambda grown in an E. coli host-deficient in adenine methylase (dam-).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")