![Dot Blot: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 4. Dot blot analysis using the antibody directed against N6-methyladenosine (m6A) To demonstrate the specificity of the antibody against N6-methyladenosine (m6A) , a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligos were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-Dot-Blot-NBP3-18690-img0001.jpg "Dot Blot: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 4. Dot blot analysis using the antibody directed against N6-methyladenosine (m6A) To demonstrate the specificity of the antibody against N6-methyladenosine (m6A) , a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligos were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | DB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.2 mg/ml |
| Description | Novus Biologicals Rabbit N6-methyladenosine (m6A) Antibody - BSA Free (NBP3-18690) is a polyclonal antibody validated for use in Dot Blot,RNA immunoprecipitation. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 ug per IP. |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide and 0.05% ProClin 300 |
| Concentration | 1.2 mg/ml |
| Purity | Protein G purified |
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reviewed by:
Yongkang Yang |
IP | Human | 07/17/2025 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for N6-methyladenosine (m6A) Antibody (NBP3-18690)Find related products by research area.
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![RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 1. RNA immunoprecipitation using the antibody directed against N6-methyladenosine (m6A) RNA Immunoprecipitation was performed on 40 ug HeLa total RNA spiked with 0.5 ug of an in vitro prepared transcript containing N6-methyladenosine (m6A) nucleotides, using 2 ug of the N6-methyladenosine (m6A) antibody . An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-RNA-immunoprecipitation-NBP3-18690-img0002.jpg "RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 1. RNA immunoprecipitation using the antibody directed against N6-methyladenosine (m6A) RNA Immunoprecipitation was performed on 40 ug HeLa total RNA spiked with 0.5 ug of an in vitro prepared transcript containing N6-methyladenosine (m6A) nucleotides, using 2 ug of the N6-methyladenosine (m6A) antibody . An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).")
![RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 3. RIP-seq results obtained with the antibody directed against N6-methyladenosine (m6A) RIP was performed with 2 ug of the antibody against N6-methyladenosine (m6A) . The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-RNA-immunoprecipitation-NBP3-18690-img0004.jpg "RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 3. RIP-seq results obtained with the antibody directed against N6-methyladenosine (m6A) RIP was performed with 2 ug of the antibody against N6-methyladenosine (m6A) . The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).")
![RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 2. RNA immunoprecipitation using the antibody directed against N6-methyladenosine (m6A) RNA Immunoprecipitation was performed on 40 ug HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 ug of antibody per RIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3 UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-RNA-immunoprecipitation-NBP3-18690-img0003.jpg "RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 2. RNA immunoprecipitation using the antibody directed against N6-methyladenosine (m6A) RNA Immunoprecipitation was performed on 40 ug HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 ug of antibody per RIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3 UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
