![Immunocytochemistry/Immunofluorescence: N6-methyladenosine (m6A) Antibody [NBP3-05657] - U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37C for the indicated time, and stained for m6A Polyclonal N6-methyladenosine (m6A)antibody, DAPI, 4', 6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel, peaking at 2 min after irradiation and diminishing over the following 8 min.](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05657-img0004.jpg "Immunocytochemistry/Immunofluorescence: N6-methyladenosine (m6A) Antibody [NBP3-05657] - U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37C for the indicated time, and stained for m6A Polyclonal N6-methyladenosine (m6A)antibody, DAPI, 4', 6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel, peaking at 2 min after irradiation and diminishing over the following 8 min.")
| Reactivity | All-MultiSpecies Glossary |
| Applications | DB, ICC/IF, IP, MA, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Rabbit N6-methyladenosine (m6A) Antibody - Azide and BSA Free (NBP3-05657) is a polyclonal antibody validated for use in ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Chemical compounds corresponding to N6-methyladenosine (m6A). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.05% Proclin 300 |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for N6-methyladenosine (m6A) Antibody (NBP3-05657)Find related products by research area.
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![N/A: N6-methyladenosine (m6A) Antibody [NBP3-05657] - N6-methyladenosine (m6A) antibody is tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).Oligomer 1 - N6-methyladenosine (m6A-CUGGUAACGAAUGGCU-G-Biotin)Oligomer 2 - unmodified adenosine (ACUGGUAACGAAUGGCU-G-Biotin)Oligomer 3 - unmodified adenosine (AAAAAAAAAAAAAAAAA-Biotin)](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-N-A-NBP3-05657-img0001.jpg "N/A: N6-methyladenosine (m6A) Antibody [NBP3-05657] - N6-methyladenosine (m6A) antibody is tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).Oligomer 1 - N6-methyladenosine (m6A-CUGGUAACGAAUGGCU-G-Biotin)Oligomer 2 - unmodified adenosine (ACUGGUAACGAAUGGCU-G-Biotin)Oligomer 3 - unmodified adenosine (AAAAAAAAAAAAAAAAA-Biotin)")
![Dot Blot: N6-methyladenosine (m6A) Antibody [NBP3-05657] - N6-methyladenosine (m6A) antibody) are tested in Dot Blot against total RNA of Arabidopsis.](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-Dot-Blot-NBP3-05657-img0002.jpg "Dot Blot: N6-methyladenosine (m6A) Antibody [NBP3-05657] - N6-methyladenosine (m6A) antibody) are tested in Dot Blot against total RNA of Arabidopsis.")
![Dot Blot: N6-methyladenosine (m6A) Antibody [NBP3-05657] - N6-methyladenosine (m6A) antibody are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.Oligomer 8 - ATAACTGG-m6A-CCGAATGGOligomer 9 - ATAACTGGACCGAATGGOligomer 10 - AAAAAAAAAAAAAAAA-biotin.](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-Dot-Blot-NBP3-05657-img0003.jpg "Dot Blot: N6-methyladenosine (m6A) Antibody [NBP3-05657] - N6-methyladenosine (m6A) antibody are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.Oligomer 8 - ATAACTGG-m6A-CCGAATGGOligomer 9 - ATAACTGGACCGAATGGOligomer 10 - AAAAAAAAAAAAAAAA-biotin.")
![Immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-05657] - Immunoprecipitation analysis of 25ug total RNA extracts of Arabidopsis (25ug total RNA extracts of Arabidopsis was diluted to 200 ul IP buffer system) using 2ul N6-methyladenosine (m6A) antibody.](http://images.novusbio.com/fullsize/N6-methyladenosine-m6A-Antibody-Immunoprecipitation-NBP3-05657-img0005.jpg "Immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-05657] - Immunoprecipitation analysis of 25ug total RNA extracts of Arabidopsis (25ug total RNA extracts of Arabidopsis was diluted to 200 ul IP buffer system) using 2ul N6-methyladenosine (m6A) antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")