IL-4 Antibodies

984-FL) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Feline IL-4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL-4 Mono-clonal Antibody (Catalog # MAB984). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox"> 984-FL) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Feline IL-4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL-4 Mono-clonal Antibody (Catalog # MAB984). The ND₅₀ is typically 1-4 µg/mL." alt="Recom-binant Feline IL-4 (Catalog # 984-FL) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Feline IL-4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL-4 Mono-clonal Antibody (Catalog # MAB984). The ND₅₀ is typically 1-4 µg/mL." class="big_thumb" />

984-FL) was serially diluted and captured by Mouse Anti-Feline IL‑4 Monoclonal Antibody (Catalog # MAB984) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Feline IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF984) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 984-FL) was serially diluted and captured by Mouse Anti-Feline IL‑4 Monoclonal Antibody (Catalog # MAB984) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Feline IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF984) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

2469-BL) (10 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Bovine IL-4 Monoclonal Antibody (Catalog # MAB2469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-4 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 2469-BL) (10 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Bovine IL-4 Monoclonal Antibody (Catalog # MAB2469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-4 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows Recombinant Bovine IL-4 (Catalog # 2469-BL) (10 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Bovine IL-4 Monoclonal Antibody (Catalog # MAB2469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-4 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

754-CL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-4 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-4 Monoclonal Antibody (Catalog # MAB754). The ND₅₀ is typically 0.4-2.0 µg/mL." class="big_lightbox"> 754-CL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-4 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-4 Monoclonal Antibody (Catalog # MAB754). The ND₅₀ is typically 0.4-2.0 µg/mL." alt="Recombinant Canine IL-4 (Catalog # 754-CL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-4 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-4 Monoclonal Antibody (Catalog # MAB754). The ND₅₀ is typically 0.4-2.0 µg/mL." class="big_thumb" />

984-FL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF984). The ND₅₀ is typically 2-8 µg/mL." class="big_lightbox"> 984-FL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF984). The ND₅₀ is typically 2-8 µg/mL." alt="Recombinant Feline IL‑4 (Catalog # 984-FL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF984). The ND₅₀ is typically 2-8 µg/mL." class="big_thumb" />

984-FL) was serially diluted and captured by Mouse Anti-Feline IL‑4 Monoclonal Antibody (Catalog # MAB984) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Feline IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF984) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 984-FL) was serially diluted and captured by Mouse Anti-Feline IL‑4 Monoclonal Antibody (Catalog # MAB984) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Feline IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF984) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

754-CL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑4 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF754). The ND₅₀ is typically 2-8 µg/mL." class="big_lightbox"> 754-CL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑4 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF754). The ND₅₀ is typically 2-8 µg/mL." alt="Recombinant Canine IL‑4 (Catalog # 754-CL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑4 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF754). The ND₅₀ is typically 2-8 µg/mL." class="big_thumb" />

504-RL) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Rat IL-4 (4 ng/mL) is neutral-ized (green line) by increasing concentrations of Mouse Anti-Rat IL-4 Monoclonal Antibody (Catalog # MAB5041). The ND₅₀ is typically 0.8-4.0 µg/mL in the presence of PHA (10 µg/mL)." class="big_lightbox"> 504-RL) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Rat IL-4 (4 ng/mL) is neutral-ized (green line) by increasing concentrations of Mouse Anti-Rat IL-4 Monoclonal Antibody (Catalog # MAB5041). The ND₅₀ is typically 0.8-4.0 µg/mL in the presence of PHA (10 µg/mL)." alt="Recombinant Rat IL-4 (Catalog # 504-RL) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Rat IL-4 (4 ng/mL) is neutral-ized (green line) by increasing concentrations of Mouse Anti-Rat IL-4 Monoclonal Antibody (Catalog # MAB5041). The ND₅₀ is typically 0.8-4.0 µg/mL in the presence of PHA (10 µg/mL)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-4 was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA using Goat Anti-Bovine IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2469) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

584-R4) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-4 (10 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-4 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF584). The ND₅₀ is typically 0.075-0.3 µg/mL in the presence of PHA (10 µg/mL)." class="big_lightbox"> 584-R4) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-4 (10 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-4 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF584). The ND₅₀ is typically 0.075-0.3 µg/mL in the presence of PHA (10 µg/mL)." alt="Recombinant Cotton Rat IL-4 (Catalog # 584-R4) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-4 (10 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-4 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF584). The ND₅₀ is typically 0.075-0.3 µg/mL in the presence of PHA (10 µg/mL)." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="IL‑4 was detected in immersion fixed paraffin-embedded sections of Mouse Spleen using Rat Anti-Mouse IL‑4 Monoclonal Antibody (Catalog # MAB4041) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />