Human Coronavirus Spike Protein Antibody (06) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity V, VSpecies Glossary
Applications WB, ELISA
Clone
06
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

Human Coronavirus Spike Protein Antibody (06) [Janelia Fluor® 549] Summary

Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Coronavirus Spike S1+S2 ECD protein.
Specificity
Recognizes the Spike of HCoV-NL63, HCoV-229E. Doesn't recognize the Spike of SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-HKU1 (isolate N5), HCoV-OC43.

Has cross-reactivity in ELISA and WB with:
HCoV-229E Spike S1+S2 ECD-His
HCoV-NL63 Spike S1+S2 ECD-His

No cross-reactivity in ELISA and WB with:
SARS-CoV-2 Spike S1-His
SARS-CoV-2 Spike S1 NTD-Fc & AVI
SARS-CoV-2 Spike S1 NTD-His & AVI
SARS-CoV-2 Spike S2 ECD-His
SARS-CoV-2 Spike RBD-His
SARS-CoV-2 Spike S1+S2 ECD-His
SARS-CoV Spike S1+S2 ECD-His
MERS-CoV Spike S1+S2 ECD-His
HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His
HCoV-OC43 Spike S1+S2 ECD-His
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
S
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Human Coronavirus Spike Protein Antibody (06) [Janelia Fluor® 549]

  • HKU1
  • Spike glycoprotein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Human Coronavirus Spike Protein Antibody (NBP3-14654JF549) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Human Coronavirus Spike Protein Products

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Bioinformatics

Gene Symbol S