![Western Blot: Human Coronavirus Spike Protein Antibody (06) [NBP3-14654] - Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-His SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-06-Western-Blot-NBP3-14654-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (06) [NBP3-14654] - Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-His SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
| Reactivity | V, VSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 06 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Immunogen | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Coronavirus Spike S1+S2 ECD protein. |
| Specificity | Recognizes the Spike of HCoV-NL63, HCoV-229E. Doesn't recognize the Spike of SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-HKU1 (isolate N5), HCoV-OC43. Has cross-reactivity in ELISA and WB with: HCoV-229E Spike S1+S2 ECD-His HCoV-NL63 Spike S1+S2 ECD-His No cross-reactivity in ELISA and WB with: SARS-CoV-2 Spike S1-His SARS-CoV-2 Spike S1 NTD-Fc & AVI SARS-CoV-2 Spike S1 NTD-His & AVI SARS-CoV-2 Spike S2 ECD-His SARS-CoV-2 Spike RBD-His SARS-CoV-2 Spike S1+S2 ECD-His SARS-CoV Spike S1+S2 ECD-His MERS-CoV Spike S1+S2 ECD-His HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His HCoV-OC43 Spike S1+S2 ECD-His |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | S |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | S |
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")