E-Selectin/CD62E Antibodies

210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA16) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> 210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA16) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " alt="Human umbilical cord endothelial cells (HUVECs) were cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA16) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

ADP1) was serially diluted and captured by Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> ADP1) was serially diluted and captured by Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

CTS013). Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="E-Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human E-Selectin/ CD62E Polyclonal Antibody (Catalog # BBA18) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA26) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> 210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA26) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " alt="Human umbilical cord endothelial cells (HUVECs) were cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA26) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

210-TA) using Mouse Anti-Human E-Selectin/CD62E Monoclonal Antibody (Catalog # BBA26) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> 210-TA) using Mouse Anti-Human E-Selectin/CD62E Monoclonal Antibody (Catalog # BBA26) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

575-ES) was serially diluted and captured by Rat Anti-Mouse E‑Selectin/CD62E Monoclonal Antibody (Catalog # MAB5751) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 575-ES) was serially diluted and captured by Rat Anti-Mouse E‑Selectin/CD62E Monoclonal Antibody (Catalog # MAB5751) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Mouse E‑Selectin/CD62E Fc Chimera (Catalog # 575-ES) was serially diluted and captured by Rat Anti-Mouse E‑Selectin/CD62E Monoclonal Antibody (Catalog # MAB5751) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />