CD74 Antibodies
We offer CD74 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Radioimmunoassay, Sandwich ELISA, Simple Western, Western Blot. Each CD74 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CD74 Antibodies can be used in a variety of model species: Baboon, Human, Mouse, Primate, Rat. Use the list below to choose the CD74 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CD74 polyclonal antibodies and browse our CD74 monoclonal antibody catalog.
Alternate Names for CD74 Antibodies
anti-CD74 antibody, anti-CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated) antibody, anti-CD74 antigen antibody, anti-CD74 molecule, major histocompatibility complex, class II invariant chain antibody, anti-DHLAGgamma chain of class II antigens antibody, anti-HLA class II histocompatibility antigen gamma chain antibody, anti-HLA-DR antigens-associated invariant chain antibody, anti-HLA-DR-gamma antibody, anti-HLADG antibody, anti-Ia antigen-associated invariant chain antibody, anti-Ia-associated invariant chain antibody, anti-Ia-GAMMA antibody, anti-Ii antibody, anti-MHC HLA-DR gamma chain antibody, anti-p33 antibody
36 Results for "cd74" in Primary Antibodies
![Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF).](http://images.novusbio.com/fullsize/CD74-Antibody-PIN-1-Western-Blot-NB100-1985-img0001.jpg "Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF).")
![Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human spleen using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/CD74-Antibody-PIN-1-Immunohistochemistry-Paraffin-NB100-1985-img0004.jpg)
![Flow Cytometry: CD74 Antibody (PIN.1) [NB100-1985] - CD74 in human PBMCs (gated on all monocytes). Orange represents isotype control and blue represents anti-CD74 antibody staining.](http://images.novusbio.com/fullsize/CD74-Antibody-PIN-1-Flow-Cytometry-NB100-1985-img0006.jpg)
![Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Ramos whole cell protein was separated on a 4-12% gel by SDS-PAGE and protein transferred to PVDF. The membrane was probed with anti-CD74 at 2 ug/ml and detected with and anti-mouse HRP secondary antibody using chemiluminescence. The arrow head shows the detection of CD74.](http://images.novusbio.com/fullsize/CD74-Antibody-PIN-1-Western-Blot-NB100-1985-img0005.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human renal cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/CD74-Antibody-PIN-1-Immunohistochemistry-Paraffin-NB100-1985-img0002.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/CD74-Antibody-PIN-1-Immunohistochemistry-Paraffin-NB100-1985-img0003.jpg)
![Western Blot: CD74 Antibody [NBP1-33109] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CD74-Antibody-Western-Blot-NBP1-33109-img0007.jpg "Western Blot: CD74 Antibody [NBP1-33109] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: CD74 Antibody [NBP1-33109] - Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD74 stained by CD74 antibody [N1N2], N-term diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/CD74-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33109-img0008.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-33109] - Human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD74-Antibody-Immunohistochemistry-Paraffin-NBP1-33109-img0003.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-33109] - Human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD74-Antibody-Immunohistochemistry-Paraffin-NBP1-33109-img0001.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-33109] - Human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD74-Antibody-Immunohistochemistry-Paraffin-NBP1-33109-img0002.jpg)
![Western Blot: CD74 Antibody [NBP1-33109] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (NBP1-33109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33109_rabbit-polyclonal-cd74-antibody-910202420122098.jpg)
![Western Blot: CD74 Antibody (LN-2) [NBP2-29465] - Western blot analysis of Raji cell lysate using CD74 antibody (LN-2).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Western-Blot-NBP2-29465-img0007.jpg "Western Blot: CD74 Antibody (LN-2) [NBP2-29465] - Western blot analysis of Raji cell lysate using CD74 antibody (LN-2).")
![Immunocytochemistry/Immunofluorescence: CD74 Antibody (LN-2) [NBP2-29465] - Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Immunocytochemistry-Immunofluorescence-NBP2-29465-img0003.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465] - Formalin-fixed, paraffin-embedded human Spleen stained with CD74 Antibody (LN-2).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Immunohistochemistry-Paraffin-NBP2-29465-img0005.jpg)
![Flow Cytometry: CD74 Antibody (LN-2) [NBP2-29465] - Flow Cytometric Analysis of Human Raji cells using CD74 Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Flow-Cytometry-NBP2-29465-img0006.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Antibody (LN-2).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Immunohistochemistry-Paraffin-NBP2-29465-img0004.jpg)
![Western Blot: CD74 Antibody (1H3) [NBP2-46179] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD74.](http://images.novusbio.com/fullsize/CD74-Antibody-1H3-Western-Blot-NBP2-46179-img0008.jpg "Western Blot: CD74 Antibody (1H3) [NBP2-46179] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD74.")
![Immunohistochemistry: CD74 Antibody (1H3) [NBP2-46179] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-1H3-Immunohistochemistry-NBP2-46179-img0007.jpg)
![Immunohistochemistry: CD74 Antibody (1H3) [NBP2-46179] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-1H3-Immunohistochemistry-NBP2-46179-img0001.jpg)
![Immunohistochemistry: CD74 Antibody (1H3) [NBP2-46179] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-1H3-Immunohistochemistry-NBP2-46179-img0002.jpg)
![Immunohistochemistry: CD74 Antibody (1H3) [NBP2-46179] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-1H3-Immunohistochemistry-NBP2-46179-img0003.jpg)
![Immunohistochemistry: CD74 Antibody (1H3) [NBP2-46179] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-1H3-Immunohistochemistry-NBP2-46179-img0004.jpg)
![Immunohistochemistry: CD74 Antibody (1H3) [NBP2-46179] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-1H3-Immunohistochemistry-NBP2-46179-img0005.jpg)
![Immunohistochemistry: CD74 Antibody (1H3) [NBP2-46179] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-1H3-Immunohistochemistry-NBP2-46179-img0006.jpg)
![Western Blot: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD74.](http://images.novusbio.com/fullsize/CD74-Antibody-OTI1H3-Azide-and-BSA-Free-Western-Blot-NBP2-70382-img0001.jpg "Western Blot: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD74.")
![Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-OTI1H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70382-img0007.jpg)
![Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-OTI1H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70382-img0002.jpg)
![Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-OTI1H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70382-img0003.jpg)
![Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-OTI1H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70382-img0004.jpg)
![Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-OTI1H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70382-img0005.jpg)
![Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-OTI1H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70382-img0006.jpg)
![Immunohistochemistry: CD74 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70382] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CD74-Antibody-OTI1H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70382-img0008.jpg)
![Western Blot: CD74 Antibody [NBP1-85225] - Analysis in human cell line Daudi.](http://images.novusbio.com/fullsize/CD74-Antibody-Western-Blot-NBP1-85225-img0005.jpg "Western Blot: CD74 Antibody [NBP1-85225] - Analysis in human cell line Daudi.")
![Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-85225] - Staining of human tonsil shows strong membranous and cytoplasmic positivity in germinal center cells and in non-germinal center cells.](http://images.novusbio.com/fullsize/CD74-Antibody-Immunohistochemistry-Paraffin-NBP1-85225-img0018.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-85225] - Staining of human cerebral cortex shows strong cytoplasmic positivity in microglia.](http://images.novusbio.com/fullsize/CD74-Antibody-Immunohistochemistry-Paraffin-NBP1-85225-img0015.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-85225] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/CD74-Antibody-Immunohistochemistry-Paraffin-NBP1-85225-img0016.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-85225] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/CD74-Antibody-Immunohistochemistry-Paraffin-NBP1-85225-img0017.jpg)
![Flow Cytometry: CD74 Antibody (CerCLIP.1) [NBP1-19249] - A cell surface stain was performed on Daudi cells with CD74 Antibody (CerCLIP.1) NBP1-19249AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD74-Antibody-CerCLIP-1-Flow-Cytometry-NBP1-19249-img0001.jpg "Flow Cytometry: CD74 Antibody (CerCLIP.1) [NBP1-19249] - A cell surface stain was performed on Daudi cells with CD74 Antibody (CerCLIP.1) NBP1-19249AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Western Blot: CD74 Antibody (2D1B3) [NBP2-37341] - Western blot analysis using CD74 mouse mAb against Raji cell lysate.](http://images.novusbio.com/fullsize/CD74-Antibody-2D1B3-Western-Blot-NBP2-37341-img0004.jpg "Western Blot: CD74 Antibody (2D1B3) [NBP2-37341] - Western blot analysis using CD74 mouse mAb against Raji cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD74 Antibody (2D1B3) [NBP2-37341] - Immunofluorescence analysis of Hela cells using CD74 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD74-Antibody-2D1B3-Immunocytochemistry-Immunofluorescence-NBP2-37341-img0005.jpg)
![Flow Cytometry: CD74 Antibody (2D1B3) [NBP2-37341] - Flow cytometric analysis of Jurkat cells using CD74 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CD74-Antibody-2D1B3-Flow-Cytometry-NBP2-37341-img0006.jpg)
![Western Blot: CD74 Antibody (2D1B3) [NBP2-37341] - Western blot analysis using CD74 mAb against human CD74 recombinant protein. (Expected MW is 37.6 kDa)](http://images.novusbio.com/fullsize/CD74-Antibody-2D1B3-Western-Blot-NBP2-37341-img0002.jpg)
![Western Blot: CD74 Antibody (2D1B3) [NBP2-37341] - Western blot analysis using CD74 mAb against HEK293 (1) and CD74 (AA: 1-106)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD74-Antibody-2D1B3-Western-Blot-NBP2-37341-img0003.jpg)
![ELISA: CD74 Antibody (2D1B3) [NBP2-37341] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD74-Antibody-2D1B3-ELISA-NBP2-37341-img0001.jpg)
![Western Blot: CD74 Antibody (CLIP/813) [NBP2-44990] - Western Blot Analysis of Raji cell lysate using CD74 antibody (CLIP/813)](http://images.novusbio.com/fullsize/CD74-Antibody-CLIP-813-Western-Blot-NBP2-44990-img0005.jpg "Western Blot: CD74 Antibody (CLIP/813) [NBP2-44990] - Western Blot Analysis of Raji cell lysate using CD74 antibody (CLIP/813)")
![Immunocytochemistry/Immunofluorescence: CD74 Antibody (CLIP/813) [NBP2-44990] - Immunofluorescent staining of Raji cells. CD74 Antibody (CLIP/813) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD74-Antibody-CLIP-813-Immunocytochemistry-Immunofluorescence-NBP2-44990-img0002.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody (CLIP/813) [NBP2-44990] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Antibody (CLIP/813).](http://images.novusbio.com/fullsize/CD74-Antibody-CLIP-813-Immunohistochemistry-Paraffin-NBP2-44990-img0003.jpg)
![Flow Cytometry: CD74 Antibody (CLIP/813) [NBP2-44990] - Flow Cytometric Analysis of Human Raji cells. CD74 Antibody (CLIP/813) followed by goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).](http://images.novusbio.com/fullsize/CD74-Antibody-CLIP-813-Flow-Cytometry-NBP2-44990-img0004.jpg)
![Western Blot: CD74 Antibody (LN-2) - Chimeric [NBP3-12079] - Daudi cell lysate (A), human spleen (B) and human tonsil (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of LN-2 (NBP3-12079) at 0.0001 ug/ml, 0.001 ug/ml and 0.0001 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Chimeric-Western-Blot-NBP3-12079-img0001.jpg "Western Blot: CD74 Antibody (LN-2) - Chimeric [NBP3-12079] - Daudi cell lysate (A), human spleen (B) and human tonsil (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of LN-2 (NBP3-12079) at 0.0001 ug/ml, 0.001 ug/ml and 0.0001 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CD74 Antibody (LN-2) - Chimeric [NBP3-12079] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of LN-2 (NBP3-12079) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12079, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12079-img0002.jpg)
![Flow Cytometry: CD74 Antibody (LN-2) - Chimeric - Azide and BSA Free [NBP3-12079] - Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12079 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12079_rabbit-monoclonal-cd74-antibody-ln-2-chimeric-7420252226527.png)