CD25/IL-2R alpha Antibodies

202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL-2 Ra Polyclonal Antibody (Catalog # AB-223-NA). The ND₅₀ is typically 5-20 µg/mL." class="big_lightbox"> 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL-2 Ra Polyclonal Antibody (Catalog # AB-223-NA). The ND₅₀ is typically 5-20 µg/mL." alt="Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL-2 Ra Polyclonal Antibody (Catalog # AB-223-NA). The ND₅₀ is typically 5-20 µg/mL." class="big_thumb" />

F0126) and Allophycocyanin-conjugated Anti-Canine CD4. Quadrant markers were set based on control antibody staining (Catalog # 5-001-A)." class="big_lightbox"> F0126) and Allophycocyanin-conjugated Anti-Canine CD4. Quadrant markers were set based on control antibody staining (Catalog # 5-001-A)." alt="Canine peripheral blood monocytes were stained with Sheep Anti-Canine CD25/IL-2 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6227) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126) and Allophycocyanin-conjugated Anti-Canine CD4. Quadrant markers were set based on control antibody staining (Catalog # 5-001-A)." class="big_thumb" />

HAF008). A specific band was detected for CD25/IL-2R alpha at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for CD25/IL-2R alpha at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB10201) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CD25/IL-2R alpha at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

202-IL,20 ng/mL) and TGF-beta (100-B,10 ng/mL) for 24 hours, then stained with (A) Rabbit anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB10203) or (B) Rabbit IgG control antibody (MAB1050), followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (F0110) and Mouse anti-Human CD3 APC-conjugated Monoclonal Antiobdy (FAB100A). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> 202-IL,20 ng/mL) and TGF-beta (100-B,10 ng/mL) for 24 hours, then stained with (A) Rabbit anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB10203) or (B) Rabbit IgG control antibody (MAB1050), followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (F0110) and Mouse anti-Human CD3 APC-conjugated Monoclonal Antiobdy (FAB100A). View our protocol for Staining Membrane-associated Proteins." alt="Human PBMCs were treated with Recombinant Human IL-2 (202-IL,20 ng/mL) and TGF-beta (100-B,10 ng/mL) for 24 hours, then stained with (A) Rabbit anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB10203) or (B) Rabbit IgG control antibody (MAB1050), followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (F0110) and Mouse anti-Human CD3 APC-conjugated Monoclonal Antiobdy (FAB100A). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

BT202, 100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha. (A) Binding is completely blocked by 2.5 µg/mL of Human Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB10203) but not by (B) Rabbit IgG Isotype Control (MAB1050) at the same concentration. Ligand binding was detected by staining cells with APC-conjugated Streptavidin (F0050)." class="big_lightbox"> BT202, 100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha. (A) Binding is completely blocked by 2.5 µg/mL of Human Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB10203) but not by (B) Rabbit IgG Isotype Control (MAB1050) at the same concentration. Ligand binding was detected by staining cells with APC-conjugated Streptavidin (F0050)." class="big_thumb" />

202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB2231R). The ND50 is typically 0.2-1.2 µg/mL." class="big_lightbox"> 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB2231R). The ND50 is typically 0.2-1.2 µg/mL." alt="Recombinant Human IL-2 (202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB2231R). The ND50 is typically 0.2-1.2 µg/mL." class="big_thumb" />

202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB10202). The ND₅₀ is typically 0.2-2.0 µg/mL." class="big_lightbox"> 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB10202). The ND₅₀ is typically 0.2-2.0 µg/mL." alt="Recombinant Human IL-2 (202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB10202). The ND₅₀ is typically 0.2-2.0 µg/mL." class="big_thumb" />