5-hmC Antibodies
We offer 5-hmC Antibodies for use in common research applications: Dot Blot, Methylated DNA Immunoprecipitation. Each 5-hmC Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our 5-hmC Antibodies can be used in a variety of model species: Human. Use the list below to choose the 5-hmC Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our 5-hmC polyclonal antibodies and browse our 5-hmC monoclonal antibody catalog.
Alternate Names for 5-hmC Antibodies
8 Results for "5-hmc" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: 5-hmC Antibody (RM236) [NBP2-61469] - Immunocytochemical staining of HeLa cells using NBP2-61469 at 0.5 ug/ml (red) . Actin filaments was labeled with fluorescein phalloidin green). HeLa cells were fixed with 4% paraformaldehyde and permeabilized with methanol (-20C) before treatment with 2 N HCl for 30 min at 37C to denature the DNA.](http://images.novusbio.com/fullsize/5-hmC-Antibody-RM236-Immunocytochemistry-Immunofluorescence-NBP2-61469-img0010.jpg "Immunocytochemistry/Immunofluorescence: 5-hmC Antibody (RM236) [NBP2-61469] - Immunocytochemical staining of HeLa cells using NBP2-61469 at 0.5 ug/ml (red) . Actin filaments was labeled with fluorescein phalloidin green). HeLa cells were fixed with 4% paraformaldehyde and permeabilized with methanol (-20C) before treatment with 2 N HCl for 30 min at 37C to denature the DNA.")
![Immunohistochemistry: 5-hmC Antibody (RM236) [NBP2-61469] - Immunohistochemical staining of formalin fixed and paraffin embedded Human brain tissue sections, using NBP2-61469](http://images.novusbio.com/fullsize/5-hmC-Antibody-RM236-Immunohistochemistry-NBP2-61469-img0009.jpg)
![Flow Cytometry: 5-hmC Antibody (RM236) [NBP2-61469] - Flow Cytometry analysis of 5-hmC expression in HEK293 cells using NBP2-61470. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with NBP2-61469 (Blue) or with a negative control antibody (red)](http://images.novusbio.com/fullsize/5-hmC-Antibody-RM236-Flow-Cytometry-NBP2-61469-img0014.jpg)
![ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - Direct ELISA of Mouse brain genomic DNA using Anti-5-hmC antibody (NBP2-61469). The plate was directly coated with different concentrations of genomic DNA isolated from Mouse brain tissue. NBP2-61469 was used as the primary antibody, and a HRP conjugated Anti-Rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/5-hmC-Antibody-RM236-ELISA-NBP2-61469-img0002.jpg)
![ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - ELISA of single stranded DNA using Anti-5-hmC antibody (NBP2-61469). The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of NBP2-61469 was used as the primary antibody, and an alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/5-hmC-Antibody-RM236-ELISA-NBP2-61469-img0011.jpg)
![ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - Direct ELISA of Mouse brain genomic DNA using Anti-5-hmC antibody (NBP2-61469). The plate was directly coated with different concentrations of genomic DNA isolated from Mouse brain tissue. NBP2-61469 was used as the primary antibody, and a HRP conjugated Anti-Rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/5-hmC-Antibody-RM236-ELISA-NBP2-61469-img0012.jpg)
![Dot Blot: 5-hmC Antibody (RM236) [NBP2-61469] - Dot blot of double stranded DNA using NBP2-61469. The membrane was pre-spotted with double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA.](http://images.novusbio.com/fullsize/5-hmC-Antibody-RM236-Dot-Blot-NBP2-61469-img0013.jpg)
![Methylated DNA Immunoprecipitation: 5-hmC Antibody (RM236) [NBP2-61469] - hMeDIP was performed using Anti-5-hmC antibody (NBP2-61469) at a 10:1 DNA: Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.](http://images.novusbio.com/fullsize/5-hmC-Antibody-RM236-Methylated-DNA-Immunoprecipitation-NBP2-61469-img0015.jpg)
![Methylated DNA Immunoprecipitation: 5-hmC Antibody (C.15220001) [NBP2-59178] - Hydroxymethylated DNA IP (hMeDIP) assays were performed using the antibody against 5-hydroxymethylcytosine, 5-hmC, 5-mC and cytosine DNA standards and Rat IgG. DNA fragments are 300-500 bp. 1 ug of mouse ES cells DNA was spiked with 0.025 ng of each DNA standard. The IP'd material has been analyzed by qPCR using the primer pairs specific to the control sequences. The obtained results are as follows: - hMeDIP on unmethylated control, with Rat IgG as negative control (0.06%, almost no recovery), with 5-hmC antibody (0.61%, almost no recovery) - hMeDIP on methylated control, with Rat IgG as negative control (0.03%, almost no recovery), with 5-hmC antibody (0.62%, almost no recovery) - hMeDIP on hydroxymethylated control, with Rat IgG as negative control (0.04%, almost no recovery), with 5-hmC (97.60% recovery, almost full recovery).](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-C-15220001-Methylated-DNA-Immunoprecipitation-NBP2-59178-img0002.jpg "Methylated DNA Immunoprecipitation: 5-hmC Antibody (C.15220001) [NBP2-59178] - Hydroxymethylated DNA IP (hMeDIP) assays were performed using the antibody against 5-hydroxymethylcytosine, 5-hmC, 5-mC and cytosine DNA standards and Rat IgG. DNA fragments are 300-500 bp. 1 ug of mouse ES cells DNA was spiked with 0.025 ng of each DNA standard. The IP'd material has been analyzed by qPCR using the primer pairs specific to the control sequences. The obtained results are as follows: - hMeDIP on unmethylated control, with Rat IgG as negative control (0.06%, almost no recovery), with 5-hmC antibody (0.61%, almost no recovery) - hMeDIP on methylated control, with Rat IgG as negative control (0.03%, almost no recovery), with 5-hmC antibody (0.62%, almost no recovery) - hMeDIP on hydroxymethylated control, with Rat IgG as negative control (0.04%, almost no recovery), with 5-hmC (97.60% recovery, almost full recovery).")
![ELISA: 5-hmC Antibody (C.15220001) [NBP2-59178] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against 5-hmC in antigen coated wells. The antigen used was a 5-hmC base coupled to KHL. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,000.](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-C-15220001-ELISA-NBP2-59178-img0001.jpg)
![Dot Blot: 5-hmC Antibody (C.15220001) [NBP2-59178] - (A): Approximately 200 ng, equivalent 10 pmol of C-bases, of the hmC (1), mC (2) and C (3) PCR controls were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 5-hmC Antibody (C.15220001) followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed for 30 seconds.](http://images.novusbio.com/fullsize/5-hmC-Antibody-C-15220001-Dot-Blot-NBP2-59178-img0004.jpg)
![Dot Blot: 5-hmC Antibody (C.15220001) [NBP2-59178] - 200 to 2 ng (equivalent of 10 to 0.1 pmol of C-base) of the hmC (1), mC (2) and C (3) PCR controls were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 4 ug/ml (dilution 1:500) of the 5-hmC Antibody (C.15220001), followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed for 30 seconds.](http://images.novusbio.com/fullsize/5-hmC-Antibody-C-15220001-Dot-Blot-NBP2-59178-img0005.jpg)
![Dot Blot: 5-hmC Antibody [NBP2-59184] - To demonstrate the specificity of the antibody against 5-hmC, a Dot blot analysis was performed using hmC, mC and C controls. One hundred to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane (Amersham Hybond-N+). The antibody was used at a dilution of 1:1,000. Figure shows a high specificity of the antibody for the hydroxymethylated control.](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-Dot-Blot-NBP2-59184-img0001.jpg "Dot Blot: 5-hmC Antibody [NBP2-59184] - To demonstrate the specificity of the antibody against 5-hmC, a Dot blot analysis was performed using hmC, mC and C controls. One hundred to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane (Amersham Hybond-N+). The antibody was used at a dilution of 1:1,000. Figure shows a high specificity of the antibody for the hydroxymethylated control.")
![Methylated DNA Immunoprecipitation: 5-hmC Antibody [NBP2-59184] - hMeDIP (hydroxymethylated DNA IP) was performed using the antibody against 5-hydroxymethylcytosine. DNA from mouse ES cells was prepared and sonicated to obtain DNA fragments of 300-500 bp. One ug of sheared DNA was spiked with the unmethylated (C) methylated (mC), and hydroxymethylated (hmC) controls. hMeDIP was performed with 3.5 ug of the rabbit 5-hmC antibody and the IP'd DNA was analysed by qPCR using primers specific for the 3 different control sequences. Figure shows that the rabbit polyclonal antibody against 5-hmC is highly specific for the 5-hmC base modification (no IP with non-methylated or methylated C control fragments).](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-Methylated-DNA-Immunoprecipitation-NBP2-59184-img0003.jpg)
![ELISA: 5-hmC Antibody [NBP2-59184] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against 5-hmC, crude serum and flow through, in antigen coated wells. The antigen used was the 5-hmC base coupled to BSA. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,800.](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-ELISA-NBP2-59184-img0002.jpg)
![Dot Blot: 5-hmC Antibody (C.15200200) [NBP2-59177] - 5-Hydroxymethylcytosine Antibody (C.15200200) [NBP2-59177] - Dot Blot analysis of the antibody with the C, mC and hmC PCR controls 200 to 2 ng (equivalent of 10 to 0.1 pmol of C-bases) of the hmC (1), mC (2) and C (3) PCR controls from the antibody (dilution 1:500). The membranes were exposed for 30 seconds.](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-C-15200200-Dot-Blot-NBP2-59177-img0001.jpg "Dot Blot: 5-hmC Antibody (C.15200200) [NBP2-59177] - 5-Hydroxymethylcytosine Antibody (C.15200200) [NBP2-59177] - Dot Blot analysis of the antibody with the C, mC and hmC PCR controls 200 to 2 ng (equivalent of 10 to 0.1 pmol of C-bases) of the hmC (1), mC (2) and C (3) PCR controls from the antibody (dilution 1:500). The membranes were exposed for 30 seconds.")
![Methylated DNA Immunoprecipitation: 5-hmC Antibody (C.15200200) [NBP2-59177] - 5-Hydroxymethylcytosine Antibody (C.15200200) [NBP2-59177] - An hydroxymethylated DNA IP (hMeDIP) was performed using the antibody directed against 5-hydroxymethylcytosine. The IgG isotype antibodies from mouse was used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated to have DNA fragments of 300-500 bp. 1 ug of human HeLa cells DNA were spiked with non-methylated, methylated, and hydroxymethylated PCR fragments. The IP'd material has been analysed by qPCR using the primer pair specific for the 3 different control sequences. The obtained results show that the mouse monoclonal for 5-hmC is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments).](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-C-15200200-Methylated-DNA-Immunoprecipitation-NBP2-59177-img0002.jpg)
![ELISA: 5-hmC Antibody (C.15200200) [NBP2-59177] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against 5-hmC in antigen coated wells. The antigen used was KHL coupled to 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000.](http://images.novusbio.com/fullsize/5-hmC-Antibody-C-15200200-ELISA-NBP2-59177-img0004.jpg)
![Dot Blot: 5-hmC Antibody (513) [NBP2-43568] - Analysis of anti- 5-Hydroxymethylcytosine / 5-hmC antibody [513] with DNA samples DNA samples (50 to 500 ng as indicated) were spotted onto positively charged nylon membrane and blotted with 5-Hydroxymethylcytosine / 5-hmC antibody [513] at a dilution of 1:500. A: Genomic DNA derived from 293T cells. B: Genomic DNA derived from human TET-1 transfected 293T cells.](http://images.novusbio.com/fullsize/5-hmC-Antibody-513-Dot-Blot-NBP2-43568-img0001.jpg "Dot Blot: 5-hmC Antibody (513) [NBP2-43568] - Analysis of anti- 5-Hydroxymethylcytosine / 5-hmC antibody [513] with DNA samples DNA samples (50 to 500 ng as indicated) were spotted onto positively charged nylon membrane and blotted with 5-Hydroxymethylcytosine / 5-hmC antibody [513] at a dilution of 1:500. A: Genomic DNA derived from 293T cells. B: Genomic DNA derived from human TET-1 transfected 293T cells.")
![Dot Blot: 5-hmC Antibody (513) [NBP2-43568] - Analysis of anti-5-hmC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine.](http://images.novusbio.com/fullsize/5-hmC-Antibody-513-Dot-Blot-NBP2-43568-img0002.jpg)
![Methylated DNA Immunoprecipitation: 5-hmC Antibody (513) [NBP2-43568] - Analysis of Human genomic DNA (500 ng) mixed with methylated cytosine standard kit, using 5-Hydroxymethylcytosine/ 5-hmC antibody [513] at 1:500 dilution. Analysized with semi-quantitative PCR and Immunprecipitated DNA was plotted as % of input DNA.](http://images.novusbio.com/fullsize/5-hmC-Antibody-513-Methylated-DNA-Immunoprecipitation-NBP2-43568-img0004.jpg)
![Dot Blot: 5-hmC Antibody [NBP2-43569] - Analysis of anti-5-hmC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody at a dilution of 1:50000. A: Unmethylated DNA B: DNA containing 5-methylcytosine C: DNA containing 5-hydroxymethylcytosine D: DNA containing 5-formylcytosine E: DNA containing 5-carboxylcytosine.](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-Dot-Blot-NBP2-43569-img0005.jpg "Dot Blot: 5-hmC Antibody [NBP2-43569] - Analysis of anti-5-hmC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody at a dilution of 1:50000. A: Unmethylated DNA B: DNA containing 5-methylcytosine C: DNA containing 5-hydroxymethylcytosine D: DNA containing 5-formylcytosine E: DNA containing 5-carboxylcytosine.")
![Methylated DNA Immunoprecipitation: 5-hmC Antibody [NBP2-43569] - Human genomic DNA (150 ng) mixed with methylated cytosine standard kit were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody at dilution of 1:2000 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-Methylated-DNA-Immunoprecipitation-NBP2-43569-img0004.jpg)