5-hmC Antibody (RM236)

Images

 
Immunocytochemistry/ Immunofluorescence: 5-hmC Antibody (RM236) [NBP2-61469] - Immunocytochemical staining of HeLa cells using Anti-5-hmC antibody (NBP2-61469, red) . Actin filaments was labeled with fluorescein ...read more
Immunohistochemistry: 5-hmC Antibody (RM236) [NBP2-61469] - Immunohistochemical staining of formalin fixed and paraffin embedded Human brain tissue sections, using Rabbit monoclonal Anti-5-hmC (NBP2-61469) antibody.
Flow Cytometry: 5-hmC Antibody (RM236) [NBP2-61469] - Flow Cytometry analysis of 5-hmC expression in HEK293 cells using NBP2-61470. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, ...read more
ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - Direct ELISA of Mouse brain genomic DNA using Anti-5-hmC antibody (NBP2-61469). The plate was directly coated with different concentrations of genomic DNA isolated from Mouse ...read more
ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - ELISA of single stranded DNA using Anti-5-hmC antibody (NBP2-61469). The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, ...read more
ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - Direct ELISA of Mouse brain genomic DNA using Anti-5-hmC antibody (NBP2-61469). The plate was directly coated with different concentrations of genomic DNA isolated from Mouse ...read more
Dot Blot: 5-hmC Antibody (RM236) [NBP2-61469] - Dot blot of double stranded DNA using Anti-5-hmC antibody (NBP2-61469). The membrane was pre-spotted with double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, ...read more
Methylated DNA Immunoprecipitation: 5-hmC Antibody (RM236) [NBP2-61469] - hMeDIP was performed using Anti-5-hmC antibody (NBP2-61469) at a 10:1 DNA: Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or ...read more

Product Details

Summary
Reactivity VbSpecies Glossary
Applications DB, ELISA, Flow, ICC/IF, IHC, IHC-P, MeDIP
Clone
RM236
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

5-hmC Antibody (RM236) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
BSA-conjugated 5-hmC
Specificity
This antibody reacts to 5-hmC in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot 0.2 ug/ml - 1 ug/ml
  • ELISA 0.1ug/ml-1ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 0.5 ug/ml - 2 ug/ml
  • Immunohistochemistry .1ug/ml - 1ug/ml
  • Immunohistochemistry-Paraffin
  • Methylated DNA Immunoprecipitation 0.2 ug/ml - 2ug/ml

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
50% Glycerol/PBS with 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for 5-hmC Antibody (RM236)

  • 5'-hmC
  • 5-hydroxymethylcytosine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

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