5-hmC Antibody (AB3/63.3) [Alexa Fluor® 750]


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Product Details

Reactivity AllSpecies Glossary
Applications ICC/IF
Alexa Fluor 750

5-hmC Antibody (AB3/63.3) [Alexa Fluor® 750] Summary

5-Hydroxymethylcytosine conjugated to BSA
Evaluated by Dot Blot in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.Dot Blot Analysis: 10 ug/mL and 1 ug/mL of this antibody detected 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.Non-species specific antibody against modified DNA. Expected to detect 5-hydroxymethylcytosine in all species.
IgG2a Kappa
Protein G purified
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  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Protein G purified


Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for 5-hmC Antibody (AB3/63.3) [Alexa Fluor® 750]

  • 5'-hmC
  • 5-hydroxymethylcytosine


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

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Alexa Fluor 594 NBP2-50099AF594
Alexa Fluor 647 NBP2-50099AF647
Alexa Fluor 700 NBP2-50099AF700
Alexa Fluor 750 NBP2-50099AF750
Biotin NBP2-50099B
DyLight 350 NBP2-50099UV
DyLight 405 NBP2-50099V
DyLight 488 NBP2-50099G
DyLight 550 NBP2-50099R
DyLight 594 NBP2-50099DL594
DyLight 650 NBP2-50099C
DyLight 680 NBP2-50099FR
DyLight 755 NBP2-50099IR
Janelia Fluor 549 NBP2-50099JF549
Janelia Fluor 646 NBP2-50099JF646

Additional 5-hmC Products

Array NBP2-50099AF750

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