5-hmC Antibody

Dot Blot: 5-hmC Antibody [NBP2-43569] - Analysis of anti-5-hmC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody at a dilution of 1:50000. A: Unmethylated DNA B: DNA containing 5-methylcytosine C: DNA containing 5-hydroxymethylcytosine D: DNA containing 5-formylcytosine E: DNA containing 5-carboxylcytosine.

Methylated DNA Immunoprecipitation: 5-hmC Antibody [NBP2-43569] - Human genomic DNA (150 ng) mixed with methylated cytosine standard kit were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody at dilution of 1:2000 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

• Reactivity: All-NA
• Applications: DB, IP, MeDIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

5-hmC Antibody Summary

• Immunogen: The immunogen used to generate this antibody corresponds to 5-HydroxymethylCytosine / 5-hmC
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100 - 1:2000
  • Immunoprecipitation
  • Methylated DNA Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for 5-hmC Antibody

• 5'-hmC
• 5-hydroxymethylcytosine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.