5-hmC Antibody (AB3/63.3)

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Product Details

Summary
Reactivity AllSpecies Glossary
Applications DB, ICC/IF, IP
Clone
AB3/63.3
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1 mg/ml

5-hmC Antibody (AB3/63.3) Summary

Immunogen
5-Hydroxymethylcytosine conjugated to BSA
Specificity
Evaluated by Dot Blot in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.Dot Blot Analysis: 10 ug/mL and 1 ug/mL of this antibody detected 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.Non-species specific antibody against modified DNA. Expected to detect 5-hydroxymethylcytosine in all species.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Publications
Read Publications using NBP2-50099.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Notes

Positive control(s): Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.

Alternate Names for 5-hmC Antibody (AB3/63.3)

  • 5'-hmC
  • 5-hydroxymethylcytosine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-Hydroxymethylcytosine Antibody (NBP2-50099)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for 5-Hydroxymethylcytosine Antibody (NBP2-50099) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-50099AF350
Alexa Fluor 405 NBP2-50099AF405
Alexa Fluor 488 NBP2-50099AF488
Alexa Fluor 532 NBP2-50099AF532
Alexa Fluor 594 NBP2-50099AF594
Alexa Fluor 647 NBP2-50099AF647
Alexa Fluor 700 NBP2-50099AF700
Alexa Fluor 750 NBP2-50099AF750
Biotin NBP2-50099B
DyLight 350 NBP2-50099UV
DyLight 405 NBP2-50099V
DyLight 488 NBP2-50099G
DyLight 550 NBP2-50099R
DyLight 594 NBP2-50099DL594
DyLight 650 NBP2-50099C
DyLight 680 NBP2-50099FR
DyLight 755 NBP2-50099IR
Janelia Fluor 549 NBP2-50099JF549
Janelia Fluor 646 NBP2-50099JF646

Additional 5-hmC Products

Array NBP2-50099

Blogs on 5-hmC

There are no specific blogs for 5-hmC, but you can read our latest blog posts.
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Contact Information

Learn the difference between western blot and simple western

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Calculators

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