5-hmC Antibody (AB3/63.3) - BSA Free

• Reactivity: All
• Applications: DB, ICC/IF, IP
• Clone: AB3/63.3
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

5-hmC Antibody (AB3/63.3) - BSA Free Summary

• Description: Novus Biologicals Rat 5-hmC Antibody (AB3/63.3) - BSA Free (NBP2-50099) is a monoclonal antibody validated for use in ICC/IF and IP. Anti-5-hmC Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: 5-hmC conjugated to BSA
• Specificity: Expected to detect 5-hydroxymethylcytosine in all species.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: Positive control(s): Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for 5-hmC Antibody (AB3/63.3) - BSA Free

• 5'-hmC
• 5-hydroxymethylcytosine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-Hydroxymethylcytosine Antibody (NBP2-50099)(5)

Publications using NBP2-50099

• M Kusi, M Zand, LL Lin, M Chen, A Lopez, CL Lin, CM Wang, ND Lucio, NB Kirma, J Ruan, TH Huang, K Mitsuya 2-Hydroxyglutarate destabilizes chromatin regulatory landscape and lineage fidelity to promote cellular heterogeneity Cell Reports, 2022-01-11;38(2):110220. 2022-01-11 [PMID: 35021081]
• Zhang Q, Lei X, Lu H. Alterations of epigenetic signatures in hepatocyte nuclear factor 4 alpha deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays. PLoS One [PMID: 24427299] (IP)
• Cao Z, Zhou N, Zhang Y et al. Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis. Theriogenology. [PMID: 24315686]
• Ficz et al. Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation. Nature [PMID: 21460836] (ICC/IF)
• Wossidlo et al. 5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming. Nat Commun. [PMID: 21407207]